Department of Orthopaedic Surgery/PSSB, Wilford Hall Medical Center, 2200 Bergquist Dr., Suite 1, 78236-5300, Lackland AFB, TX, USA.
Cytotechnology. 1996 Jan;22(1-3):263-7. doi: 10.1007/BF00353947.
The care of patients with a skeletal deficiency currently involves the use of bone graft or a non-biologic material such as a metal or polymer. There are alternate possibilities in development which involve the growth of bone cells (osteoblasts) on degradable polymer scaffolds. These tissue engineering strategies require production of the polymeric scaffold, cellular harvest followed by either ex vivo or in vivo growth of the cells on the scaffold, and exploration of the interaction between the cell and scaffold. Research into these strategies utilizes cells from a variety of species, but clinical applications will likely require human osteoblasts. This study explores the process whereby human osteoblasts are harvested under sterile conditions during joint replacement surgery from normally discarded cancellous bone, transported from the operating room to the lab, and grown in culture. This process is feasible, and the cells express their phenotype via the production of alkaline phosphatase and collagen in culture.
目前，骨骼缺陷患者的护理涉及使用骨移植物或非生物材料，如金属或聚合物。目前正在开发替代方案，涉及在可降解聚合物支架上生长骨细胞（成骨细胞）。这些组织工程策略需要生产聚合物支架、细胞收获，然后在支架上进行体外或体内细胞生长，并探索细胞与支架之间的相互作用。对这些策略的研究利用了来自多种物种的细胞，但临床应用可能需要人成骨细胞。本研究探讨了在关节置换手术中无菌条件下从通常丢弃的松质骨中收获人成骨细胞的过程，将细胞从手术室运送到实验室，并在培养中生长。这个过程是可行的，细胞通过在培养中产生碱性磷酸酶和胶原蛋白来表达其表型。
