Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, 305-701, Taejon, Korea.
Cytotechnology. 1995 Jun;19(2):153-9. doi: 10.1007/BF00749770.
The effect of serum type and concentration on recombinant protein expression in vaccinia virus infected HeLa S3 cells was studied in both static and suspension culture. A model heterologous protein,β-galactosidase (β-gal), was used. Calf and horse sera in the range of 0.5-10%(v/v) were investigated. In static culture, the calf serum concentration did not show any significant influence on the β-gal production which was almost completed within 24h postinfection (pi). Higher horse serum concentration, on the other hand, resulted in higher β-gal concentration which continued to increase until 48 h pi. Total β-gal concentrations in 0.5% calf serum at 24 h pi and 10% horse serum at 48 h pi were 2.2±0.7 and 2.2±0.1 IU/ml, respectively. In suspension culture, both sera showed their respective effects on the β-gal production similar to those observed in static culture, indicating that the cultivation method had little influence on β-gal production. Accordingly, the use of 0.5% calf serum after virus infection in recommended for economical β-gal production.
在静止和悬浮培养条件下，研究了血清类型和浓度对痘苗病毒感染的 HeLa S3 细胞中重组蛋白表达的影响。使用了一种模型异源蛋白β-半乳糖苷酶（β-gal）。研究了 0.5-10%（v/v）的小牛和马血清。在静止培养中，小牛血清浓度对β-gal 的产生没有明显影响，感染后 24 小时内几乎完成。另一方面，较高的马血清浓度导致β-gal 浓度更高，直到感染后 48 小时仍持续增加。感染后 24 小时的 0.5%小牛血清和 48 小时的 10%马血清中的总β-gal 浓度分别为 2.2±0.7 和 2.2±0.1 IU/ml。在悬浮培养中，两种血清对β-gal 的产生均表现出与静态培养相似的作用，表明培养方法对β-gal 的产生影响不大。因此，建议在病毒感染后使用 0.5%小牛血清，以经济地生产β-gal。
