使用化学酶合成的含有 C9 修饰的唾液酸的唾液酸苷来探究唾液酸酶的底物特异性。
Probe sialidase substrate specificity using chemoenzymatically synthesized sialosides containing C9-modified sialic acid.
机构信息
Department of Chemistry, University of California, One Shields Avenue, Davis, California 95616, USA.
出版信息
Chem Commun (Camb). 2012 Apr 4;48(27):3357-9. doi: 10.1039/c2cc17393j. Epub 2012 Feb 23.
PMID:22361713
Abstract
A library of α2-3- and α2-6-linked sialyl galactosides containing C9-modified sialic acids was synthesized from C6-modified mannose derivatives using an efficient one-pot three-enzyme system. These sialosides were used in a high-throughput sialidase substrate specificity assay to elucidate the importance of C9-OH in sialidase recognition.
摘要
使用高效的一锅三步酶反应体系，从 C6 位修饰的甘露糖衍生物合成了含有 C9 修饰唾液酸的α2-3-和α2-6-连接的唾液酰半乳糖苷文库。这些唾液酸用于高通量唾液酸酶底物特异性测定，以阐明 C9-OH 在唾液酸酶识别中的重要性。
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