对硝基苯酚（pNP）标记的α2-8-唾液酸苷的化学酶法合成及α2-8-唾液酸酶的高通量底物特异性研究
Chemoenzymatic synthesis of para-nitrophenol (pNP)-tagged α2-8-sialosides and high-throughput substrate specificity studies of α2-8-sialidases.
作者信息
Tasnima Nova, Yu Hai, Li Yanhong, Santra Abhishek, Chen Xi
机构信息
Department of Chemistry, University of California, One Shields Avenue, Davis, CA 95616, USA.
出版信息
Org Biomol Chem. 2016 Dec 20;15(1):160-167. doi: 10.1039/c6ob02240e.
Abstract
para-Nitrophenol (pNP)-tagged α2-8-linked sialosides containing different sialic acid forms were chemoenzymatically synthesized using an efficient one-pot three-enzyme α2-8-sialylation system. The resulting compounds allowed high-throughput substrate specificity studies of the α2-8-sialidase activity of a recombinant human cytosolic sialidase hNEU2 and various bacterial sialidases. The sialoside substrate profiles obtained can be used to guide the selection of suitable sialidases for sialylglycan analysis and for cell and tissue surface glycan modification. They can also be used to guide sialidase inhibitor design.
摘要
使用高效的一锅三酶α2-8-唾液酸化系统化学酶法合成了含有不同唾液酸形式的对硝基苯酚（pNP）标记的α2-8连接唾液酸苷。所得化合物可用于对重组人胞质唾液酸酶hNEU2和各种细菌唾液酸酶的α2-8-唾液酸酶活性进行高通量底物特异性研究。获得的唾液酸苷底物谱可用于指导选择适合唾液酸聚糖分析以及细胞和组织表面聚糖修饰的唾液酸酶。它们还可用于指导唾液酸酶抑制剂的设计。
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