Nishino Tomohiro, Sasaki Nobuya, Nagasaki Ken-Ichi, Ichii Osamu, Kon Yasuhiro, Agui Takashi
Laboratory of Laboratory Animal Science and Medicine, Department of Disease Control, Veterinary Medicine, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo, Hokkaido 060-0818.
Biomed Res. 2012 Feb;33(1):53-6. doi: 10.2220/biomedres.33.53.
The ICGN mouse strain is a glomerulosclerosis (GS) model that shows significant proteinuria, podocyte morphological abnormalities and increased extracellular matrix accumulation in the glomeruli, which represent the final common pathology associated with a variety of kidney diseases leading to end-stage renal failure. Previously, we demonstrated that GS in ICGN mice can be attributed to the deletion mutation of the tensin2 (Tns2) gene (Tns2(nep)). Further, the C57BL/6J (B6) mouse is resistant to GS caused by this mutation. 129/Sv is also a popular strain; however, its susceptibility to GS has not been defined. Thus, to determine whether 129/Sv is resistant or susceptible to GS, we produced a congenic strain carrying Tns2(nep) on the 129(+Ter)/Sv (129T) background. 129T congenic mice (129T-Tns2(nep)) did not exhibit albuminuria, renal anemia, increases in BUN, or any severe pathological changes until at least 16 weeks of age. These results indicate that 129T is resistant to GS. Although their usage in biomedical studies is already widespread, 129/Sv mice may afford a late-onset and unique strain applicable to kidney disease research.
ICGN小鼠品系是一种肾小球硬化（GS）模型，表现出显著的蛋白尿、足细胞形态异常以及肾小球细胞外基质积累增加，这些代表了与导致终末期肾衰竭的多种肾脏疾病相关的最终共同病理特征。此前，我们证明ICGN小鼠的GS可归因于张力蛋白2（Tns2）基因的缺失突变（Tns2(nep)）。此外，C57BL/6J（B6）小鼠对由该突变引起的GS具有抗性。129/Sv也是一种常用品系；然而，其对GS的易感性尚未明确。因此，为了确定129/Sv对GS是抗性还是易感，我们培育了一种在129(+Ter)/Sv（129T）背景上携带Tns2(nep)的近交系。129T近交系小鼠（129T-Tns2(nep)）在至少16周龄之前未表现出蛋白尿、肾性贫血、血尿素氮升高或任何严重的病理变化。这些结果表明129T对GS具有抗性。尽管129/Sv小鼠在生物医学研究中的应用已经很广泛，但它们可能是一种适用于肾脏疾病研究的迟发性且独特的品系。
