Ahmad Nisar, Fazal Hina, Abbasi Bilal Haider, Anwar Shazma, Basir Abdul
Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Pakistan.
Toxicol Ind Health. 2013 Jun;29(5):460-7. doi: 10.1177/0748233712436637. Epub 2012 Feb 23.
Silybum marianum L. is medicinally important for its active principle component silymarin. Silymarin regenerates damaged hepatic tissues. On the basis of such regenerative properties, the radical scavenging activity (1,1-diphenyl-2-picrylhydrazyl (DPPH)) of different tissues and the phenotypic difference of the hepatoprotective species, S. marianum L. were evaluated. There was less phenotypic difference in purple and white varieties of S. marianum. Assay of the antioxidant potential of different parts of the plant revealed that significantly higher activity (78.2%) was observed in seeds of the purple flowering plant than seeds of white flowering plant (49%) after different time intervals. Young leaves collected from white flowering plant exhibit 64.8% activity, which is higher than the purple flowering plant (55.1%). Significantly, same activity was observed in mature leaves of white (52%) and purple flowering plants (50%). The main stem collected from both the varieties exhibits similar activity from 50 to 52%. A 67.2% activity was recorded for mature roots of white flowering plant followed by roots of the purple variety (65%). The present study revealed that seeds and roots of both the varieties scavenge and detoxify more DPPH free radicals than other plant parts and can be used as a source of natural antioxidants and food additives.
水飞蓟因其活性成分水飞蓟素而具有重要药用价值。水飞蓟素可使受损的肝组织再生。基于这种再生特性，对水飞蓟不同组织的自由基清除活性（1,1-二苯基-2-苦基肼自由基（DPPH））以及具有保肝作用的水飞蓟品种的表型差异进行了评估。水飞蓟的紫色和白色品种之间的表型差异较小。对该植物不同部位的抗氧化潜力进行测定后发现，在不同时间间隔后，紫色开花植物种子的活性（78.2%）显著高于白色开花植物种子（49%）。从白色开花植物采集的幼叶活性为64.8%，高于紫色开花植物（55.1%）。值得注意的是，白色开花植物成熟叶（52%）和紫色开花植物成熟叶（50%）的活性相同。两个品种的主茎活性相似，在50%至52%之间。白色开花植物成熟根的活性为67.2%，其次是紫色品种的根（65%）。本研究表明，两个品种的种子和根比其他植物部位清除和解毒DPPH自由基的能力更强，可作为天然抗氧化剂和食品添加剂的来源。
