Flores Gema, Dastmalchi Keyvan, Dabo Abdoulaye J, Whalen Kathleen, Pedraza-Peñalosa Paola, Foronjy Robert F, D'Armiento Jeanine M, Kennelly Edward J
Department of Biological Sciences, Lehman College and The Graduate Center, City University of New York, 250 Bedford Park Boulevard West, Bronx, NY 10468, United States of America.
Food Chem. 2012 Mar 1;131(1):119-125. doi: 10.1016/j.foodchem.2011.08.044.
Four flavone C-glycosides, isoorientin (1), orientin (2), vitexin (3), and isovitexin (4), were isolated from the neotropical blueberry of Anthopterus wardii, a so-called "superfruit", using antioxidant activity-guided fractionation. A dose-response relationship of compounds 1-4 was determined for their anti-inflammatory activity against interleukin-8 (IL-8) and for the inhibition of matrix metalloproteinase-1 (MMP-1) expression, an inflammatory marker for chronic obstructive pulmonary disease. The four flavone C-glycosides exhibited inhibitory activity against IL-8 production and MMP-1 expression, with compounds 1, 3, and 4 having the most potent inhibitory activities in both assays at 100 μg/ml. The structures of compounds 1-4 were determined by spectroscopic methods. These flavone C-glycosides are reported for the first time in the Anthopterus genus.
通过抗氧化活性导向分离法，从所谓的“超级水果”——新热带蓝莓Anthopterus wardii中分离出了四种黄酮C-糖苷，即异荭草苷（1）、荭草苷（2）、牡荆素（3）和异牡荆素（4）。测定了化合物1 - 4对白细胞介素-8（IL-8）的抗炎活性以及对基质金属蛋白酶-1（MMP-1）表达（慢性阻塞性肺疾病的一种炎症标志物）的抑制作用的剂量反应关系。这四种黄酮C-糖苷对IL-8的产生和MMP-1的表达均表现出抑制活性，在两种测定中，化合物1、3和4在100μg/ml时具有最强的抑制活性。通过光谱方法确定了化合物1 - 4的结构。这些黄酮C-糖苷首次在Anthopterus属中被报道。
