Wang Jin, Yue Yong-de, Jiang Hao, Tang Feng
SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Centre for Bamboo and Rattan, No. 8 Futong Dongdajie, Wangjing, Chaoyang District, Beijing 100102, China.
Int J Anal Chem. 2012;2012:205101. doi: 10.1155/2012/205101. Epub 2012 May 10.
A strategy for analyzing flavone C-glucosides in the leaves of different species of bamboo was developed. Firstly, the flavone C-glycosides were extracted from the bamboo leaves (51 species in 17 genera) with methanol and chromatographed on silica gel 60 plates in automatic developing chamber (ADC2), and a qualitative survey using simple derivatization steps with the NP reagent was carried out. The flavone C-glycosides were found in 40 of 51 species of bamboo examined. Secondly, an HPLC method with photodiode array and multiple wavelength detector was optimized and validated for the simultaneous determination of flavone C-glycosides, including isoorientin, isovitexin, orientin, and vitexin in the leaves of three species of bamboo and the flavone C-glycosides were confirmed by LC/MS. The optimized HPLC method proved to be linear in the concentration range tested (0.2-100 μg/mL, r(2) ≥ 0.9997), precise (RSD ≤ 1.56%), and accurate (88-106%). The concentration ranges of isoorientin, isovitexin, orientin, and vitexin in three bamboo leaves samples were 1.00-2.78, 0-0.31, 0-0.07, and 0.20-0.68 mg/g, respectively. The proposed method was validated to be simple and reliable and can be a tool for quality control of bamboo leaf extract or its commercial products.
开发了一种分析不同竹种叶片中黄酮碳苷的策略。首先，用甲醇从竹叶（17属51种）中提取黄酮碳苷，并在自动展开室（ADC2）中的硅胶60板上进行色谱分析，并用NP试剂通过简单的衍生步骤进行定性分析。在所检测的51种竹子中，有40种发现了黄酮碳苷。其次，优化并验证了一种采用光电二极管阵列和多波长检测器的高效液相色谱法，用于同时测定三种竹子叶片中的黄酮碳苷，包括异荭草苷、异牡荆苷、荭草苷和牡荆苷，并用液相色谱/质谱法对黄酮碳苷进行了确认。优化后的高效液相色谱法在所测试的浓度范围内（0.2 - 100μg/mL，r²≥0.9997）呈线性，精密度高（相对标准偏差≤1.56%），准确度高（88 - 106%）。三种竹叶样品中异荭草苷、异牡荆苷、荭草苷和牡荆苷的浓度范围分别为1.00 - 2.78、0 - 0.31、0 - 0.07和0.20 - 0.68mg/g。所提出的方法经验证简单可靠，可作为竹叶提取物或其商业产品质量控制的工具。
