Birang Reza, Torabi Alireza, Shahabooei Mohammad, Rismanchian Mansour
Department of Periodontics and Torabinejad Dental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran.
Dent Res J (Isfahan). 2012 Jan;9(1):93-9. doi: 10.4103/1735-3327.92961.
Tissue engineering principles can be exploited to enhance alveolar and peri-implant bone reconstruction by applying such biological factors as platelet-derived growth factors. The objective of the present study is to investigate the effect of autologous plasma-rich in growth factors (on the healing of peri-implant bone in canine mandible).
In this prospective experimental animal study, two healthy canines of the Iranian mix breed were selected. Three months after removing their premolar teeth on both sides of the mandible, 12 implants of the Osteo Implant Corporationsystem, 5 mm in diameter and 10 mm in length, were selected to be implanted. Plasma rich in growth factors (PRGF) were applied on six implants while the other six were used as plain implants without the plasma. The implants were installed in osteotomy sites on both sides of the mandible to be removed after 4 weeks with the surrounding bones using a trephine bur. Mesio-distal sections and implant blocks, 50 μ in diameter containing the peri-implant bone, were prepared By basic fuchin toluidine-bluefor histological and histomorphometric evaluation by optical microscope. The data were analyzed using Mann-Whitney Test (P<0.05).
The bone trabeculae and the type of bone generation in PRGF and control groups had no statistically significant differences (P=0.261, P=0.2) although the parameters showed higher measured values in the PRGF group. However, compared to the control, application of PRGF had significantly increased bone-to-implant contact (P=0.028)
Based on the results, it may be concluded that application of PRGF on the surface of implant may enhance bone-to-implant contact.
组织工程学原理可通过应用诸如血小板衍生生长因子等生物因子来促进牙槽骨和种植体周围骨的重建。本研究的目的是探讨富含生长因子的自体血浆对犬下颌种植体周围骨愈合的影响。
在这项前瞻性实验动物研究中，选取了两只伊朗混血健康犬。在拔除下颌两侧的前磨牙三个月后，选择12枚直径5毫米、长度10毫米的Osteo Implant Corporation系统种植体进行植入。将富含生长因子的血浆（PRGF）应用于6枚种植体，而另外6枚作为未使用血浆的普通种植体。将种植体植入下颌两侧的截骨部位，4周后使用环钻去除种植体及其周围的骨组织。制备直径50微米、包含种植体周围骨的近远中切片和种植体块，用碱性品红甲苯胺蓝染色，通过光学显微镜进行组织学和组织形态计量学评估。使用Mann-Whitney检验分析数据（P<0.05）。
尽管PRGF组的各项参数测量值较高，但PRGF组和对照组的骨小梁及骨生成类型无统计学显著差异（P=0.261，P=0.2）。然而，与对照组相比，应用PRGF显著增加了骨与种植体的接触（P=0.028）。
基于结果可以得出结论，在种植体表面应用PRGF可能会增强骨与种植体的接触。
