Peng Wang, Kim Il-Kyu, Cho Hyun-Young, Seo Ji-Hoon, Lee Dong-Hwan, Jang Jun-Min, Park Seung-Hoon
Department of Oral and Maxillofacial Surgery, College of Medicine, Inha University, Incheon, South Korea.
Department of OMFS, Dentistry, College of Medicine, Inha University, #7-206, 3rd St. Shinheung-dong, Choong-gu, Incheon, 400-711 South Korea.
Maxillofac Plast Reconstr Surg. 2016 Mar 24;38(1):16. doi: 10.1186/s40902-016-0061-5. eCollection 2016 Dec.
The association of biomaterial combined with repair factor-like platelet-rich plasma (PRP) has prospective values. Bovine-derived xenograft has been identified as an osteoconductive and biocompatible grafting material that provides osseointegration ability. PRP has become a valuable adjunctive agent to promote healing in a lot of dental and oral surgery procedures. However, there are controversies with respect to the regenerative capacity of PRP and the real benefits of its use in bone grafts. The purpose of this study was to assess the influence of PRP combined with xenograft for the repair of peri-implant bone defects.
Twelve rabbits were used in this study, and the experimental surgery with implant installation was performed simultaneously. Autologous PRP was prepared before the surgical procedure. An intrabony defect (7.0 mm in diameter and 3.0 mm deep) was created in the tibia of each rabbit; then, 24 titanium dental implants (3.0 mm in diameter and 8.5 mm long) were inserted into these osteotomy sites. Thus, a standardized gap (4.0 mm) was established between the surrounding bony walls and the implant surface. The gaps were treated with either xenograft alone (control group) or xenograft combined with PRP (experimental group). After healing for 1, 2, 3, 4, 5, and 6 weeks, the rabbits were sacrificed with an overdose of KCl solution. Two rabbits were killed at each time, and the samples including dental implants and surrounding bone were collected and processed for histological analysis.
More newly formed bone and a better bone healing process were observed in control group. The histomorphometric analysis revealed that the mean percentage of bone-to-implant contact in the control group was significantly higher than that of the experimental group (25.23 vs. 8.16 %; < 0.05, independent-simple test, analysis of variance [ANOVA]).
The results indicate that in the addition of PRP to bovine-derived xenograft in the repair of bone defects around the implant, PRP may delay peri-implant bone healing.
生物材料与修复因子样富血小板血浆（PRP）联合应用具有潜在价值。牛源异种移植物已被确认为一种具有骨传导性和生物相容性的移植材料，具备骨整合能力。PRP已成为促进许多牙科和口腔外科手术愈合的一种有价值的辅助剂。然而，关于PRP的再生能力及其在骨移植中使用的实际益处存在争议。本研究的目的是评估PRP联合异种移植物对种植体周围骨缺损修复的影响。
本研究使用12只兔子，同时进行植入种植体的实验性手术。在手术前制备自体PRP。在每只兔子的胫骨上制造一个骨内缺损（直径7.0毫米，深3.0毫米）；然后，将24颗牙科钛种植体（直径3.0毫米，长8.5毫米）插入这些截骨部位。这样，在周围骨壁与种植体表面之间建立了一个标准化的间隙（4.0毫米）。这些间隙分别用单独的异种移植物（对照组）或异种移植物联合PRP（实验组）进行处理。在愈合1、2、3、4、5和6周后，用过量的氯化钾溶液处死兔子。每次处死2只兔子，收集包括牙科种植体和周围骨的样本并进行组织学分析。
在对照组中观察到更多新形成的骨和更好的骨愈合过程。组织形态计量学分析显示，对照组骨与种植体接触的平均百分比显著高于实验组（25.23%对8.16%；P<0.05，独立样本t检验，方差分析[ANOVA]）。
结果表明，在植入物周围骨缺损修复中，在牛源异种移植物中添加PRP可能会延迟种植体周围骨愈合。
