Department of Parasitology, Faculty of Veterinary Medicine, University of Firat, 23119 Elazig, Turkey.
Vet Parasitol. 2012 Jul 6;187(3-4):567-71. doi: 10.1016/j.vetpar.2012.01.035. Epub 2012 Feb 3.
The study reports the frequency of infestation and the prevalence of tick-borne pathogens in feeding adult ticks detached from cattle in two climatic zones of the Black Sea region of Turkey. A total of 2160 adult ticks were collected during 2007-2008. Of these, 1062 were randomly selected, divided into 224 pools, and tested for the presence of bovine Theileria, Babesia, and Anaplasma species. Eleven tick species were recognized on cattle in the study. Hyalomma marginatum was widely disrubuted in the semi-arid bioclimatic zone, but few specimens were collected in the humid bioclimatic zone. The most prevalent tick species in the humid climatic zone was Ixodes ricinus. Infection rates were calculated as the maximum likelihood estimation with 95% confidence intervals (CI). Overall, 4% (CI 2.87-5.44) of 224 tick pools were found to be positive for the pathoges by Reverse line blot. Maximum likelihood estimation of the infection rate varied among tick species, ranging from 2.68% (CI 0.16-12.68) in Haemaphysalis sulcata to 10.49% (CI 4.07-23.66) in Rhipicephalus bursa. The most prevalent tick-borne pathogen was Anaplasma phagocytophilum at 6.78% (CI 3.41-12.18) followed by A. centrale (6.56%, CI 0.42-31.47), Anaplasma/Ehrlichia spp. (3.61%, CI 1.99-6.06), Babesia spp. (3.33%, CI 1.65-6.03), and T. buffeli/orientalis (2.71%, CI 0.73-7.18). Sequencing results indicated that Babesia spp. shared 99% to 100% similarity with the unnamed Babesia sp. Kashi 1 and 2, Babesia sp. Kayseri 1 and Babesia sp.CS58. Anaplasma/Ehrlichia spp. were 98% and 100% identical to Ehrlichia canis and Ehrlichia sp. Omatjenne strain, respectively.
本研究报告了在土耳其黑海地区两个气候带中，从牛身上取下的寄生成年蜱的寄生频率和蜱传病原体的流行率。在 2007 年至 2008 年间共采集了 2160 只成年蜱。其中，随机选择了 1062 只，分为 224 个池，并检测了牛的泰勒虫、巴贝虫和无形体属的存在情况。在研究中，在牛身上识别出了 11 种蜱种。在半干旱生物气候带中，边缘革蜱分布广泛，但在潮湿生物气候带中采集到的标本很少。在潮湿气候带中最流行的蜱种是蓖子硬蜱。感染率通过反向线印迹以 95%置信区间（CI）进行最大似然估计计算。总体而言，224 个蜱池中有 4%（CI 2.87-5.44）通过反向线印迹呈病原体阳性。在不同的蜱种中，感染率的最大似然估计值有所不同，从硬蜱的 2.68%（CI 0.16-12.68）到璃眼蜱的 10.49%（CI 4.07-23.66）不等。最流行的蜱传病原体是嗜吞噬细胞无形体，感染率为 6.78%（CI 3.41-12.18），其次是中央无形体（6.56%，CI 0.42-31.47）、无形体/埃立克体属（3.61%，CI 1.99-6.06）、巴贝虫属（3.33%，CI 1.65-6.03）和水牛泰勒虫/东方泰勒虫（2.71%，CI 0.73-7.18）。测序结果表明，巴贝虫属与未命名的喀什 1 号和 2 号巴贝虫、开塞利 1 号巴贝虫和 CS58 号巴贝虫共享 99%至 100%的相似性。无形体/埃立克体属与犬埃立克体和奥马滕内恩株埃立克体分别有 98%和 100%的一致性。
