Centro de Microscopía Electrónica, Universidad Nacional de Córdoba, Haya de la Torre esq, Enrique Barros, Ciudad Universitaria, CP 5000 Córdoba, Argentina.
Mol Cell Endocrinol. 2012 May 15;355(1):169-79. doi: 10.1016/j.mce.2012.02.008. Epub 2012 Feb 15.
In the present work we aimed at identifying ERα in the plasma membrane of normal anterior pituitary cells and investigated if 17β-estradiol was able to induce their subcellular redistribution. Our results show that about 8% of anterior pituitary cells expressed ERα in the plasma membrane, with the geometrical mean fluorescence intensity being increased after steroid hormone treatment. 17β-Estradiol and the selective ERα agonist PPT induced an increase of ERα expression in the plasma membrane and activated the PKCα/ERK 1/2 pathway in a time-course not compatible with genomic actions, thus supporting the notion of membrane-initiated effects. These findings suggest that 17β-estradiol stimulates the translocation of endogenous ERα to the plasma membrane, consequently modulating this ER pool and leading to cellular biological effects in normal anterior pituitary gland.
在本工作中，我们旨在鉴定正常垂体前叶细胞浆膜中的 ERα，并研究 17β-雌二醇是否能够诱导其亚细胞重新分布。我们的结果表明，约 8%的垂体前叶细胞在浆膜中表达 ERα，类固醇激素处理后其几何平均荧光强度增加。17β-雌二醇和选择性 ERα激动剂 PPT 诱导 ERα在浆膜中的表达增加，并激活 PKCα/ERK1/2 途径，其时间进程与基因组作用不兼容，因此支持膜起始作用的概念。这些发现表明，17β-雌二醇刺激内源性 ERα向浆膜的易位，从而调节该 ER 池，并在前正常垂体中导致细胞生物学效应。
