两种方法从粪便中提取隐孢子虫 DNA 的比较，采用实时 PCR 法检测。
A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay.
机构信息
UK Cryptosporidium Reference Unit, Public Health Wales Microbiology, Singleton Hospital, Swansea, SA2 8QA, United Kingdom.
出版信息
J Microbiol Methods. 2012 Apr;89(1):38-40. doi: 10.1016/j.mimet.2012.02.006. Epub 2012 Feb 16.
PMID:22366300
Abstract
Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P=0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.
摘要
通过珠磨、硫氰酸胍和硅胶纯化直接从 46 份粪便中提取隐孢子虫 DNA 的 PCR 阳性率（93.5%对 100%）略低，阈值循环值（平均值 34.93 对 28.03；P=0.00）也更高，而煮沸、半纯化卵囊悬浮液的螺旋柱提取则更高。然而，直接提取更便宜，也更适合自动化。
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