Center for Bioresource and Bioenergy, School of Biotechnology, Jiangnan University, Wuxi, People's Republic of China.
Biotechnol Lett. 2012 Jun;34(6):1123-30. doi: 10.1007/s10529-012-0883-x. Epub 2012 Feb 25.
Coupling lactate fermentation with cell growth was investigated in shake-flask and bioreactor cultivation systems by increasing aeration to improve lactate productivity in Escherichia coli CICIM B0013-070 (ackA pta pps pflB dld poxB adhE frdA). In shake-flasks, cells reached 1 g dry wt/l then, cultivated at 100 rpm and 42°C, achieved a twofold higher productivity of lactic acid compared to aerobic and O(2)-limited two-phase fermentation. The cells in the bioreactor yielded an overall volumetric productivity of 5.5 g/l h and a yield of 86 g lactic acid/100 g glucose which were 66% higher and the same level compared to that of the aerobic and O(2)-limited two-phase fermentation, respectively, using scaled-up conditions optimized from shake-flask experiments. These results have revealed an approach for improving production of fermentative products in E. coli.
通过增加通气来提高大肠杆菌 CICIM B0013-070（ackA pta pps pflB dld poxB adhE frdA）的乳酸生产力，在摇瓶和生物反应器培养系统中研究了将乳酸发酵与细胞生长偶联。在摇瓶中，细胞达到 1 克干重/升，然后在 100 rpm 和 42°C 下培养，与好氧和 O(2)-有限两相发酵相比，乳酸的生产力提高了两倍。在生物反应器中，细胞的总体体积产率为 5.5 g/l h，乳酸产率为 86 g 乳酸/100 g 葡萄糖，与好氧和 O(2)-有限两相发酵相比，分别提高了 66%和相同水平，使用从摇瓶实验优化的放大条件。这些结果揭示了一种在大肠杆菌中提高发酵产物生产的方法。
