Blizard Institute, Barts and the London School of Medicine and Dentistry, Queen Mary University of London, Newark St, London E1 2AT, UK.
Nucleic Acids Res. 2012 Jul;40(12):5262-70. doi: 10.1093/nar/gks158. Epub 2012 Feb 25.
Activation of the major histocompatibility complex (MHC) by interferon-gamma (IFN-γ) is a fundamental step in the adaptive immune response to pathogens. Here, we show that reorganization of chromatin loop domains in the MHC is evident within the first 30 min of IFN-γ treatment of fibroblasts, and that further dynamic alterations occur up to 6 h. These very rapid changes occur at genomic sites which are occupied by CTCF and are close to IFN-γ-inducible MHC genes. Early responses to IFN-γ are thus initiated independently of CIITA, the master regulator of MHC class II genes and prepare the MHC for subsequent induction of transcription.
干扰素-γ（IFN-γ）对主要组织相容性复合体（MHC）的激活是宿主对病原体产生适应性免疫反应的一个基本步骤。在这里，我们发现，在 IFN-γ处理成纤维细胞的最初 30 分钟内，MHC 中的染色质环域发生了明显的重排，并且这种动态变化一直持续到 6 小时。这些非常迅速的变化发生在 CTCF 占据的基因组位点上，并且靠近 IFN-γ诱导的 MHC 基因。因此，IFN-γ 的早期反应独立于 MHC 类 II 基因的主调控因子 CIITA 而发生，并为随后的转录诱导做好 MHC 的准备。
