Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, The Chinese Academy of Sciences, Wuhan 430071, China.
Biochem Biophys Res Commun. 2012 Mar 23;419(4):617-20. doi: 10.1016/j.bbrc.2012.02.057. Epub 2012 Feb 17.
During infection, Staphylococcus aureus produces multiple enzymes that enable it to invade and destroy host tissues and metastasize to other sites. One such enzyme, lipase, has been recognized for its relationship in the virulence of S. aureus. However, a direct involvement of lipase in the pathogenesis of S. aureus remains to be demonstrated. Our prior study indicated that anti-lipase serum inhibits biofilm formation in S. aureus clinical strains. The aim of this study was to further characterize the roles of lipase in the pathogenesis in S. aureus. We found that deletion of the lipase-coding gene reduced biofilm formation relative to the wild-type strain. This was shown by culture in 96-well plates coated with collagen to resemble the in vivo infection process. Intraperitoneal inoculation of mice with a lipase mutant strain showed defective formation of peritoneal abscesses, and bacterial loads in different organs were much lower compared with the wild-type. Importantly, active immunization with recombinant lipase protected mice against a lethal challenge with S. aureus. Altogether, our data provide evidence that S. aureus lipase plays important roles in the pathogenesis of S. aureus.
在感染过程中，金黄色葡萄球菌会产生多种酶，使其能够侵入和破坏宿主组织，并转移到其他部位。其中一种酶，脂酶，因其与金黄色葡萄球菌的毒力有关而受到关注。然而，脂酶在金黄色葡萄球菌发病机制中的直接作用仍有待证实。我们之前的研究表明，抗脂酶血清可抑制金黄色葡萄球菌临床株的生物膜形成。本研究旨在进一步探讨脂酶在金黄色葡萄球菌发病机制中的作用。我们发现，与野生型菌株相比，脂酶编码基因缺失后生物膜形成减少。这是通过在涂有胶原蛋白的 96 孔板中培养来实现的，类似于体内感染过程。用脂酶突变株腹腔接种小鼠，显示出腹膜脓肿形成缺陷，与野生型相比，不同器官中的细菌载量要低得多。重要的是，用重组脂酶进行主动免疫可保护小鼠免受金黄色葡萄球菌致死性攻击。总之，我们的数据提供了证据，表明金黄色葡萄球菌脂酶在金黄色葡萄球菌的发病机制中发挥重要作用。
