US Army Research Institute of Environmental Medicine, Natick, MA, USA.
Physiol Meas. 2012 Mar;33(3):385-94. doi: 10.1088/0967-3334/33/3/385. Epub 2012 Feb 28.
Accurate measurement of sweat mineral loss is important for whole body mineral balance estimates and dietary reference intake formulation. Currently, common localized sweat collection methods such as the pouch and patch techniques may be limited by skin encapsulation and/or hidromeiosis, which may alter sweat mineral concentrations. The design of the newly developed Megaduct sweat collector may avoid these possible limitations. Therefore, the purpose of this study was to evaluate the utility of the Megaduct sweat collector for mineral analysis. Megaduct sweat collectors were affixed to ten volunteers on the final day of a heat acclimation protocol; collection time, sweat volume, and mineral concentrations of calcium, copper, iron, potassium, sodium, and zinc were measured. Megaduct filling required a collection period of 62 ± 3 min due to a small collection surface (22.1 cm(2)). The mineral content of the sweat was 0.3 ± 0.1 mmol L(-1), 1.5 ± 1.5 µmol L(-1), 8.5 ± 2.1 mmol L(-1), 43.2 ± 15.0 mmol L(-1), and 10.1 ± 5.7 µmol L(-1) for Ca, Cu, K, Na, and Zn, respectively. The Megaduct sweat collector appears to avoid skin encapsulation and hidromeiosis, and captures sweat with similar mineral concentrations as reported in the literature for pouches. However, the filling time of the Megaduct (>60 min) may not capture possible changes in sweat mineral concentrations that are documented to occur in as little as 15 to 30 min.
准确测量汗液中的矿物质流失量对于全身矿物质平衡评估和膳食参考摄入量的制定非常重要。目前，常见的局部汗液收集方法，如囊袋和贴片技术，可能受到皮肤包裹和/或出汗减少的限制，这可能会改变汗液中的矿物质浓度。新开发的 Megaduct 汗液收集器的设计可能会避免这些可能的限制。因此，本研究的目的是评估 Megaduct 汗液收集器在矿物质分析中的实用性。在热适应方案的最后一天，将 Megaduct 汗液收集器贴在十名志愿者身上；测量收集时间、汗液量以及钙、铜、铁、钾、钠和锌的矿物质浓度。由于收集面积较小（22.1cm²），Megaduct 收集器需要 62±3 分钟才能收集满。汗液中的矿物质含量分别为 0.3±0.1mmol/L、1.5±1.5µmol/L、8.5±2.1mmol/L、43.2±15.0mmol/L 和 10.1±5.7µmol/L，分别为钙、铜、钾、钠和锌。Megaduct 汗液收集器似乎避免了皮肤包裹和出汗减少，并且收集的汗液中的矿物质浓度与囊袋报告的文献中的浓度相似。然而，Megaduct 的填充时间（>60 分钟）可能无法捕捉到汗液矿物质浓度的可能变化，这些变化据报道在 15 到 30 分钟内就会发生。
