Pesticide Residue Analysis Laboratory, Department of Entomology, Punjab Agricultural University, Ludhiana, 141 004, Punjab, India.
Bull Environ Contam Toxicol. 2012 May;88(5):781-7. doi: 10.1007/s00128-012-0578-y. Epub 2012 Feb 28.
In the present studies potential of microorganisms isolated from the cotton fields was explored for the bioremediation of fipronil contaminated soils. The cultures of microbes were grown in Luria broth as shake culture maintained at 28°C. After that Dorn's broth enrichment culture supplemented with fipronil was used and isolated two bacterial cultures viz. Paracoccus sp. and Gamma Proteobacteria. The performance of both bacteria was evaluated for degradation of fipronil in soil. Paracoccus sp. was found better than the Gamma Proteobacteria as far as degradation of fipronil in soil is concerned. The samples of soil were extracted and cleaned up by following a standardized methodology. Fipronil was quantified by gas liquid chromatography and confirmed by gas chromatography mass spectrometer. The limit of quantification of fipronil was worked out to be 10 μg kg(-1). By using this methodology, the mean recoveries of fipronil in different types of soil were found to be more than 85%. The residues of fipronil were found to persist only up to 10 days in soils fortified with fipronil @ 20 μg kg(-1) and amended with Paracoccus sp. while in the soils fortified @ 80 μg kg(-1) fipronil, residues persisted up to 20, 30 and 30 days in loamy sand, sandy loam and clay loam, respectively. Therefore, the use of Paracoccus sp. can further be explored for the bioremediation of fipronil contaminated soils.
在本研究中，探索了从棉田分离出的微生物在生物修复氟虫腈污染土壤中的潜力。微生物的培养物在 Luria 肉汤中作为摇瓶培养物生长，保持在 28°C。之后，使用补充了氟虫腈的 Dorn 肉汤富集培养物，并分离出两种细菌培养物，即假单胞菌和γ变形菌。评估了这两种细菌在土壤中降解氟虫腈的性能。就土壤中氟虫腈的降解而言，假单胞菌的性能优于γ变形菌。通过采用标准化方法提取和净化土壤样品。使用气相色谱和气相色谱-质谱联用仪对氟虫腈进行定量。氟虫腈的定量限为 10 μg kg(-1)。使用这种方法，在不同类型的土壤中，氟虫腈的平均回收率均高于 85%。在添加了假单胞菌的情况下，用氟虫腈 @ 20 μg kg(-1) 处理的土壤中，氟虫腈的残留仅持续 10 天，而在添加了氟虫腈 @ 80 μg kg(-1)的土壤中，在砂壤土、砂壤土和粘壤土中，残留分别持续 20、30 和 30 天。因此，可以进一步探索假单胞菌的使用，以实现氟虫腈污染土壤的生物修复。
