丙戊酸可提高牛体细胞核移植胚胎的体外发育能力。
Valproic acid improves the in vitro development competence of bovine somatic cell nuclear transfer embryos.
作者信息
Xu Wenbing, Wang Yongsheng, Li Yanyan, Wang Lijun, Xiong Xianrong, Su Jianmin, Zhang Yong
机构信息
College of Veterinary Medicine, Northwest A&F University, Key Laboratory of Animal Reproductive Physiology & Embryo Technology, Ministry of Agriculture, Yangling, Shaanxi, People's Republic of China.
出版信息
Cell Reprogram. 2012 Apr;14(2):138-45. doi: 10.1089/cell.2011.0084. Epub 2012 Feb 28.
Abstract
The present study was carried out to examine the effect of valproic acid (VPA), an important histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 lysine 9 (H3K9ac) in bovine somatic cell nuclear transfer (SCNT) embryos. We found that treatment with 4 mM VPA for 24 h could significantly improve the development of bovine SCNT embryos. Compared with the no-treatment group, the cleavage rate was higher (69.79 ± 0.99% vs. 65.11 ± 1.02%, p<0.05), as was the blastocyst rate (39.99 ± 1.29% vs. 34.87 ± 1.74%, p<0.05). Moreover, the rate of apoptosis (1.91 ± 0.48% vs. 5.67 ± 0.40%, p<0.05) in blastocysts was greatly reduced after VPA treatment. Valproic acid treatment also increased the immunofluorescent signal for H3K9ac in SCNT embryos in a pattern similar to that of in vitro fertilized (IVF) embryos. In conclusion, we demonstrated that VPA can significantly improve the in vitro developmental competence and enhance the nuclear reprogramming of bovine SCNT embryos.
摘要
本研究旨在检测重要的组蛋白去乙酰化酶抑制剂丙戊酸（VPA）对牛体细胞核移植（SCNT）胚胎体外发育及表观遗传标记组蛋白H3赖氨酸9（H3K9ac）表达的影响。我们发现，用4 mM VPA处理24小时可显著改善牛SCNT胚胎的发育。与未处理组相比，其卵裂率更高（69.79 ± 0.99% 对 65.11 ± 1.02%，p<0.05），囊胚率也是如此（39.99 ± 1.29% 对 34.87 ± 1.74%，p<0.05）。此外，VPA处理后囊胚中的凋亡率大幅降低（1.91 ± 0.48% 对 5.67 ± 0.40%，p<0.05）。丙戊酸处理还使SCNT胚胎中H3K9ac的免疫荧光信号增加，其模式类似于体外受精（IVF）胚胎。总之，我们证明VPA可显著提高牛SCNT胚胎的体外发育能力并增强其核重编程。
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