Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA.
Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA; Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, 807, Taiwan.
Exp Eye Res. 2021 Oct;211:108726. doi: 10.1016/j.exer.2021.108726. Epub 2021 Aug 14.
Posterior Capsule Opacification (PCO) is the most common complication associated with Intraocular Lens (IOL) implantation. Based on the assumption that the interactions between an IOL and the lens capsule (LC) may influence the extent of PCO formation, a new in vitro model was developed to quantify the adhesion force of an IOL to simulated LC using a custom-designed micro-force tester. Using this system, we examined the influence of temperature (room temperature vs. body temperature) and incubation time (0 vs. 24 h) on the adhesion force between IOLs and LCs. The results show that, in line with clinical observations of PCO incidence, the adhesion force increased at body temperature and with increase in incubation time in the following order, Acrylic foldable IOLs > Silicone IOLs > PMMA IOLs. By examining the changes of surface properties as a function of temperature and incubation time, we found that acrylic foldable IOLs showed the largest increase in their hydrophilicity and reported the lowest surface roughness in comparison to other IOL groups. Coincidentally, using a newly established macromolecular dye imaging system to simulate cell migration between IOLs and LC, we observed that the amount of macromolecular dye infiltration between IOLs and LCs was in the following order: PMMA IOLs > Silicone IOLs > Acrylic foldable IOLs. These results support a new potential mechanism that body temperature, incubation time, surface hydrophilicity and smoothness of IOLs greatly contribute to their tight binding to LCs and such tight binding may lead to reduced IOL: LC space, cell infiltration, and thus PCO formation.
后囊混浊（PCO）是与人工晶状体（IOL）植入相关的最常见并发症。基于 IOL 与晶状体囊（LC）之间的相互作用可能影响 PCO 形成程度的假设，开发了一种新的体外模型，使用定制设计的微力测试仪来量化 IOL 与模拟 LC 之间的粘附力。使用该系统，我们研究了温度（室温与体温）和孵育时间（0 与 24 小时）对 IOL 和 LC 之间粘附力的影响。结果表明，与 PCO 发生率的临床观察一致，粘附力随着体温升高和孵育时间延长而增加，以下顺序为：丙烯酸可折叠 IOLs > 硅胶 IOLs > PMMA IOLs。通过检查表面特性随温度和孵育时间的变化，我们发现与其他 IOL 组相比，丙烯酸可折叠 IOLs 的亲水性增加最大，表面粗糙度最低。巧合的是，使用新建立的大分子染料成像系统模拟 IOL 和 LC 之间的细胞迁移，我们观察到 IOL 和 LC 之间大分子染料渗透量的顺序为：PMMA IOLs > 硅胶 IOLs > 丙烯酸可折叠 IOLs。这些结果支持了一个新的潜在机制，即体温、孵育时间、IOL 的表面亲水性和平整度极大地促进了它们与 LC 的紧密结合，这种紧密结合可能导致 IOL：LC 空间减少、细胞浸润，从而导致 PCO 形成。
