Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Sto. Tomas, 11340 DF México.
Mol Ecol Resour. 2012 Jul;12(4):590-7. doi: 10.1111/j.1755-0998.2012.03125.x. Epub 2012 Feb 28.
The mitochondrial cytochrome c oxidase subunit I gene is the standard DNA barcoding region used for species identification and discovery. We examined the variation of COI (454 bp) to discriminate 20 species of bats in the family Phyllostomidae that are found in the Yucatan Peninsula of southeastern Mexico and northern Guatemala and compared them genetically to other samples from Central America. The majority of these species had low intraspecific variation (mean = 0.75%), but some taxa had intraspecific variation ranging to 8.8%, suggesting the possibility of cryptic species (i.e. Desmodus rotundus and Artibeus jamaicensis). There was a recurring biogeographic pattern in eight species with a separation of northern and southern Middle American localities. The Yucatan Peninsula was a discrete area identified in four species, whereas Panama was recovered in five species of phyllostomid bats. Our study establishes a foundation for further molecular work incorporating broader taxonomic and geographic coverage to better understand the phylogeography and genetic diversity that have resulted from the ecological constraints in this region and the remarkable differentiation of bats in the Neotropics.
线粒体细胞色素 c 氧化酶亚基 I 基因是用于物种鉴定和发现的标准 DNA 条形码区域。我们检查了 COI（454bp）的变异，以区分在墨西哥东南部和危地马拉北部的 Yucatan 半岛发现的 20 种 Phyllostomidae 蝙蝠，并将它们与来自中美洲的其他样本进行了遗传比较。这些物种的大多数具有较低的种内变异（平均值=0.75%），但有些分类群的种内变异范围达到 8.8%，这表明可能存在隐种（即 Desmodus rotundus 和 Artibeus jamaicensis）。有 8 个物种存在反复出现的生物地理模式，其中北方和南方中美洲的种群分离。Yucatan 半岛在 4 个物种中是一个离散的区域，而巴拿马则在 5 个 Phyllostomidae 蝙蝠物种中被发现。我们的研究为进一步的分子工作奠定了基础，包括更广泛的分类和地理覆盖范围，以更好地了解该地区生态限制所导致的系统地理学和遗传多样性，以及新热带地区蝙蝠的显著分化。
