Fresh Produce Research Centre, Harper Adams University College, Newport, Shropshire, UK.
J Appl Microbiol. 2012 May;112(5):1007-19. doi: 10.1111/j.1365-2672.2012.05269.x. Epub 2012 Mar 19.
To improve our understanding of the survival and splash-mediated transfer of zoonotic agents and faecal indicator bacteria introduced into soils used for crop cultivation via contaminated irrigation waters.
Zoonotic agents and an Escherichia coli marker bacterium were inoculated into borehole water, which was applied to two different soil types in early-, mid- and late summer. Decline of the zoonotic agents was influenced by soil type. Marker bacteria applied to columns of two soil types in irrigation water did not concentrate at the surface of the soils. Decline of zoonotic agents at the surface was influenced by soil type and environmental conditions. Typically, declines were rapid and bacteria were not detectable after 5 weeks. Selective agar strips were used to determine that the impact of water drops 24-87 μl could splash marker bacteria from soil surfaces horizontal distances of at least 25 cm and heights of 20 cm.
Soil splash created by rain-sized water droplets can transfer enteric bacteria from soil to ready-to-eat crops. Persistence of zoonotic agents was reduced at the hottest part of the growing season when irrigation is most likely.
Soil splash can cause crop contamination. We report the penetration depths and seasonally influenced declines of bacteria applied in irrigation water into two soil types.
通过受污染灌溉水将引入用于作物种植的土壤中的人畜共患病原体和粪便指示菌进行原位生存和溅出转移，从而提高我们对此的理解。
将人畜共患病原体和大肠杆菌标记菌接种到钻孔水中，然后在夏初、仲夏和夏末将其应用于两种不同的土壤类型。土壤类型影响人畜共患病原体的衰减。将标记菌应用于灌溉水中两种土壤类型的柱状物中时，不会在土壤表面集中。土壤表面人畜共患病原体的衰减受土壤类型和环境条件的影响。通常，下降迅速，并且在 5 周后就无法检测到细菌。选择性琼脂条用于确定直径为 24-87μl 的水滴可将标记菌从土壤表面溅起，水平距离至少为 25cm，高度为 20cm。
由雨滴大小的水滴滴溅可将肠道细菌从土壤转移到可即食的作物上。在最有可能进行灌溉的生长季节最热的时期，人畜共患病原体的持续时间会缩短。
土壤溅出可导致作物污染。我们报告了在两种土壤类型中应用于灌溉水中的细菌的穿透深度和季节性下降情况。
