Characterization of metalloprotease and serine protease activities in batch CHO cell cultures: control of human recombinant IFN-γ proteolysis by addition of iron citrate.

作者信息

Clincke Marie-Françoise, Guedon Emmanuel, Yen Frances T, Ogier Virginie, Goergen Jean-Louis

机构信息

Laboratoire Réactions et Génie des Procédés UPR-CNRS 3349, ENSAIA-INPL, Nancy-Université, 54505 Vandoeuvre-lès-Nancy, France ; Lipidomix (EA4422), ENSAIA-INPL, Nancy-Université, 54505 Vandoeuvre-lès-Nancy, France ; Genclis SAS, 54505 Vandoeuvre-lès-Nancy, France.

Laboratoire Réactions et Génie des Procédés UPR-CNRS 3349, ENSAIA-INPL, Nancy-Université, 54505 Vandoeuvre-lès-Nancy, France.

出版信息

BMC Proc. 2011 Nov 22;5 Suppl 8(Suppl 8):P115. doi: 10.1186/1753-6561-5-S8-P115. eCollection 2011.

DOI:10.1186/1753-6561-5-S8-P115

PMID:22373384

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3285009/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f4cd/3285009/51cc982371ed/1753-6561-5-S8-P115-1.jpg

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本文引用的文献

In Vitro Cell Dev Biol Anim. 2005 Mar-Apr;41(3-4):83-91. doi: 10.1290/0411075.1.

The macroheterogeneity of recombinant human interferon-gamma produced by Chinese-hamster ovary cells is affected by the protein and lipid content of the culture medium.中国仓鼠卵巢细胞产生的重组人干扰素-γ的宏观异质性受培养基中蛋白质和脂质含量的影响。

Biotechnol Appl Biochem. 1995 Feb;21(1):87-100.

Optimisation of hybridoma cell growth and monoclonal antibody secretion in a chemically defined, serum- and protein-free culture medium.在化学成分明确、无血清和无蛋白的培养基中优化杂交瘤细胞生长和单克隆抗体分泌

J Immunol Methods. 1989 Jan 6;116(1):65-77. doi: 10.1016/0022-1759(89)90314-1.

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