Laboratório de Micologia, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
Laboratório de Investigação de Peptidases, Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Mediators Inflamm. 2017;2017:8952878. doi: 10.1155/2017/8952878. Epub 2017 Jul 26.
is a facultative intracellular opportunistic fungal pathogen in human infections. Several virulence-associated attributes are involved in its pathogenesis, host-pathogen interactions, modulation of host immune defenses, and regulation of antifungal drug resistance. This study evaluated the in vitro antifungal susceptibility profile to five antifungal agents, the production of seven hydrolytic enzymes related to virulence, and the relationship between these phenotypes in 91 clinical strains of . All strains were susceptible to flucytosine. However, some of these strains showed resistance to amphotericin B (9.9%), fluconazole (15.4%), itraconazole (5.5%), or micafungin (15.4%). Overall, strains were good producers of catalase, aspartic protease, esterase, phytase, and hemolysin. However, caseinase and phospholipase in vitro activities were not detected. Statistically significant correlations were identified between micafungin minimum inhibitory concentration (MIC) and esterase production, between fluconazole and micafungin MIC and hemolytic activity, and between amphotericin B MIC and phytase production. These results contribute to clarify some of the mechanisms of pathogenicity. Moreover, the association between some virulence attributes and the regulation of antifungal resistance encourage the development of new therapeutic strategies involving virulence mechanisms as potential targets for effective antifungal drug development for the treatment of infections.
是一种兼性细胞内机会性真菌病原体，可引起人类感染。其发病机制、宿主-病原体相互作用、宿主免疫防御的调节以及抗真菌药物耐药性的调节等多个毒力相关属性都参与其中。本研究评估了 91 株临床分离株对五种抗真菌药物的体外抗真菌药敏谱、七种与毒力相关的水解酶的产生情况以及这些表型之间的关系。所有 株均对氟胞嘧啶敏感。然而，其中一些菌株对两性霉素 B（9.9%）、氟康唑（15.4%）、伊曲康唑（5.5%）或米卡芬净（15.4%）表现出耐药性。总体而言， 株是过氧化氢酶、天冬氨酸蛋白酶、酯酶、植酸酶和溶血素的良好产生者。然而，并未检测到体外的组织蛋白酶和磷脂酶活性。米卡芬净最低抑菌浓度（MIC）与酯酶产生之间、氟康唑和米卡芬净 MIC 与溶血活性之间以及两性霉素 B MIC 与植酸酶产生之间存在显著的相关性。这些结果有助于阐明 的一些发病机制。此外，一些毒力属性与抗真菌耐药性调节之间的关联鼓励开发新的治疗策略，这些策略涉及毒力机制，作为开发有效抗真菌药物治疗 感染的潜在靶点。
