Department of Medical Chemistry, Biochemistry and Clinical Chemistry School of Medicine, University Hospital Centre, University of Zagreb, Zagreb, Croatia.
Arch Med Res. 2012 Feb;43(2):145-53. doi: 10.1016/j.arcmed.2012.02.004. Epub 2012 Feb 26.
Human obesity is accepted as an important risk factor for development of MetS. Adiponectin is linked to central obesity and ADIPOQ variants are promising markers for understanding the genetic base of obesity-related disorders. We performed analyses of adiponectin concentrations and ADIPOQ variants and tested their associations with obesity and MetS in young subjects of Croatian origin.
Biochemical and anthropometric parameters of MetS were obtained for 149 unrelated subjects. Adiponectin levels were measured by ELISA assay. ADIPOQ -11391G>A and -11377C>G were genotyped by real-time PCR.
BMI and WC, TG and GLUC showed inverse correlation, whereas HDL-C showed a positive correlation with adiponectin concentrations. For central obesity, we found association with -11377C>G and with -11391G>A polymorphisms. ADIPOQ -11377GG and -11391GA significantly increased the risk for the development of central obesity (OR 5.57 and OR 3.37, respectively). Significant association was found between -11391A, -11377G allele and haplotype and increased TG. -11377C>G and -11391G>A variant were significantly associated with the incidence of MetS. C>G mutation at position -11377 significantly increased the risk of MetS development (OR = 2.93). Compared with the -11391G homozygotes, carriers of the A allele had a significantly increased risk for the development of MetS (OR = 3.15). The test of overall association showed a statistically significant correlation of MetS with -11377C>G and -11391G>A haplotypes (p = 0.008).
Analysis of adiponectin concentration and ADIPOQ -11391G>A and -11377C>G gene variants may be clinically meaningful for estimation of MetS risk in a young population.
人体肥胖被认为是代谢综合征（MetS）发展的一个重要危险因素。脂联素与中心性肥胖有关，ADIPOQ 变异是了解肥胖相关疾病遗传基础的有希望的标志物。我们对来自克罗地亚的年轻人群进行了脂联素浓度和 ADIPOQ 变异体的分析，并测试了它们与肥胖和 MetS 的相关性。
对 149 名无血缘关系的受试者进行了代谢综合征的生化和人体测量参数分析。采用 ELISA 法测定脂联素水平。采用实时 PCR 法对 ADIPOQ-11391G>A 和-11377C>G 进行基因分型。
BMI 和 WC、TG 和 GLUC 呈负相关，而 HDL-C 与脂联素浓度呈正相关。对于中心性肥胖，我们发现与-11377C>G 和-11391G>A 多态性有关。ADIPOQ-11377GG 和-11391GA 显著增加了中心性肥胖的发病风险（OR 分别为 5.57 和 3.37）。-11391A、-11377G 等位基因和单倍型与 TG 升高显著相关。-11377C>G 和-11391G>A 变异与 MetS 的发生显著相关。-11377 位置的 C>G 突变显著增加了 MetS 发展的风险（OR=2.93）。与-11391G 纯合子相比，A 等位基因携带者发生 MetS 的风险显著增加（OR=3.15）。整体关联检验显示，MetS 与-11377C>G 和-11391G>A 单倍型具有统计学显著相关性（p=0.008）。
脂联素浓度和 ADIPOQ-11391G>A 和-11377C>G 基因变异的分析对估计年轻人群 MetS 风险可能具有临床意义。
