Department of Polymer Science and Engineering, MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University, Hangzhou 310027, People's Republic of China.
J Biomed Mater Res A. 2012 Jun;100(6):1387-97. doi: 10.1002/jbm.a.34077. Epub 2012 Feb 28.
Surface immobilization of bioactive molecules has been a promising strategy to develop in situ endothelialization for cardiovascular implants. With the aim to construct endothelial cell specific coating with low fouling property, zwitterionic carboxybetaine methacrylate and butyl methacrylate were copolymerized as coating materials, spin-coated onto substrates, and immobilized with endothelial cell selective peptide Arg-Glu-Asp-Val (REDV) through functionalization of carboxy groups in carboxybetaine by NHS/EDC chemistry. Experimental results proved that carboxybetaine-REDV coating maintained desirable antifouling ability and fine hemocompatibility. Separate culture and coculture of HUVECs (human umbilical vein endothelial cells) with HUASMCs (human umbilical artery smooth muscle cells) showed that the coating was able to enhance the competitive growth of endothelial cells while limiting the adhesion, proliferation, and migration of smooth muscle cells. The existence of zwitterionic carboxybetaine helps to screen undesirable adsorption of platelets, and its nonspecific resistance to smooth muscle cells contributes to the realization of endothelial cell selectivity.
表面固定生物活性分子是一种很有前途的策略，可以用于开发心血管植入物的原位内皮化。本研究旨在构建具有低蛋白吸附性能的内皮细胞特异性涂层，将两性离子羧基甜菜碱甲基丙烯酸酯和甲基丙烯酸丁酯共聚作为涂层材料，旋涂在基底上，并通过 NHS/EDC 化学将羧基功能化，将内皮细胞选择性肽 Arg-Glu-Asp-Val（REDV）固定在上面。实验结果证明，羧基甜菜碱-REDV 涂层保持了良好的抗污染能力和优良的血液相容性。对 HUVECs（人脐静脉内皮细胞）和 HUASMCs（人脐动脉平滑肌细胞）的单独培养和共培养表明，该涂层能够增强内皮细胞的竞争生长，同时限制平滑肌细胞的黏附、增殖和迁移。两性离子羧基甜菜碱的存在有助于筛选血小板的非特异性吸附，其对平滑肌细胞的非特异性抵抗有助于实现内皮细胞的选择性。
