Zhang Wei-Tong, Chai Dong, Xu Shan, Liu Ping, Liu Kun
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2014 Jul;30(4):352-6.
To research the change of concentration of the amino acid neurotransmitters in the striatum focal cerebral ischemia in rat and the effect of Acorus tatarinowii Schott, one of inducing resuscitation drugs, for 4 of amino acid neurotransmitters.
Twenty four rats were divided into four groups (n = 6): control group, cerebral ischemia group, sham operation group and Acorns tatarinowii Schott treated group. Rats were established into models of cerebral ischemia by occluding bilateral thread cork method. Formation sampling were performed in a striatum area using microdialysis and the detection of biological sample including aspartic acid, glutamic acid, glycine and gamma-aminobutyric acid by high performance liquid chromatography (HPLC) electrochemical detector system.
Compared with the control, the all contents of 4 kinds of the amino acids were significantly increased during cerebral ischemia (P < 0.01). Compared with the cerebral ischemia group, the contents of aspartic acid, glutamic acid that were excitatory amino acids were remarkably decreased in the striatum for Acorus tatarinowii Schott treated group (P < 0.01), It was no significant influence on gamma-aminobutyric acid and glycine that belonged to inhibitory amino acid in a nascent condition but with a elevating in the later period of microdialysis.
Acorus tatarinowii Schott can enter the cerebral parenchyma through blood brain barrier and cut down glutamic acid,aspartic acid increased during cerebral ischemia. As a result, the neurotoxicity attributed to the excitatory amino acid has been released in excessive amounts declined so as to avoid the secondary impairment of neurons caused by excitatory amino acids pernicious effects after ischemia. It may be one of the protective mechanism of drugs for inducing resuscitation resembling EAA receptor antagonists to ischemi brain.
研究大鼠纹状体局灶性脑缺血时氨基酸类神经递质浓度的变化以及醒脑开窍药石菖蒲对4种氨基酸类神经递质的影响。
将24只大鼠分为4组（n = 6）：对照组、脑缺血组、假手术组和石菖蒲治疗组。采用双侧线栓法制备大鼠脑缺血模型。运用微透析技术在纹状体区域进行活体取样，并用高效液相色谱（HPLC）电化学检测系统检测生物样品中的天冬氨酸、谷氨酸、甘氨酸和γ-氨基丁酸。
与对照组相比，脑缺血时4种氨基酸含量均显著升高（P < 0.01）。与脑缺血组相比，石菖蒲治疗组纹状体中兴奋性氨基酸天冬氨酸、谷氨酸含量显著降低（P < 0.01），对微透析前期属于抑制性氨基酸的γ-氨基丁酸和甘氨酸无明显影响，但后期有所升高。
石菖蒲可透过血脑屏障进入脑实质，降低脑缺血时升高的谷氨酸、天冬氨酸含量，从而减轻兴奋性氨基酸过量释放所导致的神经毒性，避免缺血后兴奋性氨基酸毒性作用对神经元造成的继发性损伤，这可能是其类EAA受体拮抗剂样药物对缺血脑保护机制之一。
