Rockhill R C, Lesmana M, Moechtar M A, Sutomo A
Southeast Asian J Trop Med Public Health. 1980 Dec;11(4):441-5.
Protein A-containing Staphylococcus aureus was coupled to Salmonella C1, D and Vi monovalent antisera to produce C1-, D- and Vi-COAG reagents. The reagents were used to detect their homologous Salmonella antigens in blood cultures (BC). The D and Vi antigens were detected in 79 of 239 BC from patients with suspected typhoid fever and Salmonella typhi was later isolated from the same 79 BC. The C1 antigen was detected in 8 BC from which only S. oranienburg was later isolated. The COAG test was generally positive at the same time the BC became culture positive. However, because of subculture and biochemical identification requirements the COAG test could be interpreted 1-2 days before culture results were available. The COAG test can be used to presumptively identify Salmonella typhi and Salmonella group C1 in blood cultures before the culture results are available.
将含蛋白A的金黄色葡萄球菌与沙门氏菌C1、D和Vi单价抗血清偶联，制备C1-、D-和Vi-COAG试剂。这些试剂用于检测血培养（BC）中它们的同源沙门氏菌抗原。在239份疑似伤寒患者的血培养中，有79份检测到D和Vi抗原，随后从这79份血培养中分离出伤寒沙门氏菌。在8份血培养中检测到C1抗原，随后仅从中分离出奥兰宁堡沙门氏菌。COAG试验通常在血培养呈阳性的同时呈阳性。然而，由于传代培养和生化鉴定的要求，COAG试验可在培养结果出来前1-2天进行解读。COAG试验可用于在培养结果出来之前，对血培养中的伤寒沙门氏菌和C1组沙门氏菌进行初步鉴定。
