Shetty N P, Srinivasa H, Bhat P
Am J Clin Pathol. 1985 Jul;84(1):80-4. doi: 10.1093/ajcp/84.1.80.
The efficacy of two methods--coagglutination (COAG) and counter immunoelectrophoresis (CIE)--in the rapid diagnosis of typhoid fever was studied in parallel with blood and clot cultures on 114 clinically suspected cases. Retrospective analysis showed that only 58 eventually were discharged and had typhoid fever. Antigen detection on their sera was done by both methods, concomitant with antigen detection on culture supernates by CIE. Sera from 50 controls were subjected to both tests. Agglutinating anti-serum being unsatisfactory in the CIE system, anti-serum to the LPS fraction of Salmonella typhi "O" 901 was used in both tests after absorption with Escherichia coli and Salmonella paratyphi A. Analysis of data with reference to retrospectively confirmed typhoid cases show that S. typhi was isolated in 58.6% and 58.3% of blood and clot cultures; antigen detection by CIE in their supernates was 81.1% and 79.2%, respectively. This correlated closely with serum COAG (81.0%) in contrast to serum CIE (5.7%). Thus, COAG was superior to CIE for serology. However, CIE done on culture supernates precludes such tedious procedures as absorption of staphylococcal agglutinins and the confirmatory blocking test.
在114例临床疑似伤寒热的病例中，采用协同凝集试验（COAG）和对流免疫电泳（CIE）两种方法进行快速诊断，并与血培养和血块培养同时进行研究。回顾性分析显示，最终只有58例确诊为伤寒热并出院。对这些患者的血清进行了两种方法的抗原检测，同时用CIE对培养上清液进行抗原检测。对50名对照者的血清也进行了这两种检测。由于在CIE系统中凝集抗血清效果不佳，因此在两种检测中均使用经大肠杆菌和甲型副伤寒沙门菌吸收后的伤寒沙门菌“O”901脂多糖组分抗血清。根据回顾性确诊的伤寒病例分析数据表明，血培养和血块培养中伤寒沙门菌的分离率分别为58.6%和58.3%；CIE对其培养上清液的抗原检测率分别为81.1%和79.2%。这与血清COAG（81.0%）密切相关，而与血清CIE（5.7%）形成对比。因此，在血清学检测方面，COAG优于CIE。然而，对培养上清液进行CIE检测可避免诸如葡萄球菌凝集素吸收和确证性阻断试验等繁琐程序。
