Dar Arshud, Lai Ken, Dent Donna, Potter Andrew, Gerdts Volker, Babiuk Lorne A, Mutwiri George K
Vaccine and Infectious Disease Organization, Department of Veterinary Microbiology, University of Saskatchewan, 120-Veterinary Road, Saskatoon, SK, Canada S7N 5E3.
Vet Immunol Immunopathol. 2012 May 15;146(3-4):289-95. doi: 10.1016/j.vetimm.2012.01.021. Epub 2012 Feb 6.
The selection of an optimal adjuvant to enhance the potency and longevity of antigen specific immune responses has always been imperative for the development of more effective and safer vaccines. A balanced type of immune enhancing ability of a new adjuvant known as polyphosphazene (PCEP) has been demonstrated in mice. In the present study we have compared the humoral and cellular immune responses to vaccine formulations containing Actinobacillus pleuropneumoniae outer membrane antigen (OmlA) with PCEP or Emulsigen as adjuvants. Our data showed a significant improvement and a shift toward more balanced immune responses when OmlA antigen was formulated with PCEP and CpG ODN. Moreover, in contrast to Emulsigen, immunization with PCEP did not result in local injection site reactions highlighting its potential as a safe and effective adjuvant for pigs. Further, the ease of formulation, administration and relatively low per dose cost of PCEP make it a promising adjuvant for pigs.
对于开发更有效、更安全的疫苗而言，选择一种最佳佐剂以增强抗原特异性免疫反应的效力和持久性一直至关重要。一种名为聚磷腈（PCEP）的新型佐剂在小鼠体内展现出了平衡的免疫增强能力。在本研究中，我们比较了以PCEP或乳化剂（Emulsigen）作为佐剂的含有胸膜肺炎放线杆菌外膜抗原（OmlA）的疫苗制剂所引发的体液免疫和细胞免疫反应。我们的数据表明，当OmlA抗原与PCEP和CpG ODN配制时，免疫反应有显著改善且向更平衡的免疫反应转变。此外，与乳化剂不同，用PCEP免疫不会导致局部注射部位反应，这突出了其作为猪用安全有效佐剂的潜力。此外，PCEP易于配制、给药且每剂成本相对较低，使其成为猪用佐剂的一个有前景的选择。
