Rosano T G, Brown H H
Clin Chem. 1979 Apr;25(4):550-4.
We describe a "high-performance" liquid-chromatographic procedure for measuring 3-methoxy-4-hydroxymandelic acid (vanillylmandelic acid) in urine. The compound is quantitatively extracted from urine by use of an automated system, including an anion-exchange resin. After elution from the resin and concentration by air drying, the urine extract is chromatographed on an octyl silica column, with use of a buffered mobile phase. To overcome interference from other organic acid in the extract, the column effluent is reacted with periodate in an analytical system to oxidize the analyte and internal standard to products that absorb at 360 nm. We evaluated the procedure for precision, linearity, analytical recovery, interfering substances, and correlation with an established procedure. The combination of a preliminary resin extraction, a liquid-chromatographic separation, and a periodate oxidative monitor results in a rapid, specific method that can be adapted for use in the clinical laboratory.
我们描述了一种用于测量尿中3-甲氧基-4-羟基扁桃酸（香草扁桃酸）的“高效”液相色谱法。该化合物通过使用包括阴离子交换树脂的自动化系统从尿中定量提取。从树脂上洗脱并通过空气干燥浓缩后，尿提取物在辛基硅胶柱上进行色谱分析，使用缓冲流动相。为了克服提取物中其他有机酸的干扰，在分析系统中使柱流出物与高碘酸盐反应，将分析物和内标氧化为在360nm处有吸收的产物。我们评估了该方法的精密度、线性、分析回收率、干扰物质以及与既定方法的相关性。初步树脂提取、液相色谱分离和高碘酸盐氧化监测相结合，产生了一种快速、特异的方法，可适用于临床实验室。
