Nebinger P, Koel M
Medizinisches Labor, Dr. Enzenauer & Dr. Wilhelm, Osnabrück, F.R.G.
J Chromatogr. 1988 Dec 29;434(1):43-9. doi: 10.1016/0378-4347(88)80060-4.
A new rapid high-performance liquid chromatographic method, without urine sample pre-treatment and based on isocratic ion-pair elution, fluorimetric detection and column-switching, has been developed for the determination of vanillylmandelic acid. The sensitivity was 0.1 mg/l, and the linearity was excellent in the concentration range tested. For all endogenous substances as well as for all the drugs tested no interferences were observed. Typical concentrations were in the range 0.3-5.5 mg of vanillylmandelic acid per day, depending on the age of subject under investigation.
已开发出一种新型快速高效液相色谱法，该方法无需对尿液样本进行预处理，基于等度离子对洗脱、荧光检测和柱切换技术，用于测定香草扁桃酸。灵敏度为0.1毫克/升，在所测试的浓度范围内线性良好。对于所有内源性物质以及所有测试药物，均未观察到干扰。根据所研究对象的年龄，香草扁桃酸的典型浓度范围为每天0.3 - 5.5毫克。
