Bondia-Pons Isabel, Boqué Noemí, Paternain Laura, Santamaría Enrique, Fernández Joaquín, Campión Javier, Milagro Fermín, Corrales Fernando, Martínez J Alfredo
Department of Nutrition, Food Science, Physiology and Toxicology, University of Navarra, Pamplona, Spain.
J Nutrigenet Nutrigenomics. 2011;4(6):344-53. doi: 10.1159/000336075. Epub 2012 Feb 25.
BACKGROUND/AIMS: The aim of this study was to gain insight into those proteins that might be involved in the early stages of liver fat accumulation as a consequence of a different fat versus simple sugar dietary intake.
Forty-five male Wistar rats were randomly distributed into four dietary groups: a starch-rich control diet (CD; n = 10), a high-fat diet (n = 12), a high-sucrose diet (n = 11), and a high-fat sucrose diet (HFSD; n = 12) for 5 weeks. A comparative analysis by 2D-DIGE and LC-ESI-MS/MS was performed to characterize the liver protein expression profiles due to the three obesogenic diets.
Ten out of 17 proteins whose expression levels were altered by >1.25-fold were identified. Four proteins (Hspa8, Hspa9, Ca3, and Cat) were differentially expressed after the HFSD period compared to CD. The heat shock proteins (Hspa8 and Hspa9) resulted significantly downregulated in liver from rats fed HFSD versus CD (p < 0.05). The results were confirmed by Western blot.
This descriptive study might be useful for further studies aiming at understanding the mechanisms by which diets rich in both fat and sugar affect the initiation of hepatic steatosis.
背景/目的：本研究旨在深入了解因不同脂肪与单糖饮食摄入导致肝脏脂肪积累早期阶段可能涉及的蛋白质。
将45只雄性Wistar大鼠随机分为四组饮食组：富含淀粉的对照饮食组（CD；n = 10）、高脂饮食组（n = 12）、高蔗糖饮食组（n = 11）和高脂高蔗糖饮食组（HFSD；n = 12），持续5周。通过二维差异凝胶电泳（2D-DIGE）和液相色谱-电喷雾串联质谱（LC-ESI-MS/MS）进行比较分析，以表征三种致肥胖饮食导致的肝脏蛋白质表达谱。
鉴定出17种表达水平变化超过1.25倍的蛋白质中的10种。与CD相比，HFSD喂养期后有4种蛋白质（Hspa8、Hspa9、Ca3和Cat）差异表达。与CD组相比，HFSD喂养大鼠肝脏中的热休克蛋白（Hspa8和Hspa9）显著下调（p < 0.05）。蛋白质印迹法证实了该结果。
这项描述性研究可能有助于进一步研究旨在了解富含脂肪和糖的饮食影响肝脂肪变性起始的机制。
