Center for Optical Diagnostics and Therapy, Department of Radiation Oncology, Erasmus Medical Center, Rotterdam 3000 CA, The Netherlands.
Opt Lett. 2012 Mar 1;37(5):948-50. doi: 10.1364/OL.37.000948.
This study utilizes Monte Carlo simulations of single fiber fluorescence to develop an empirical model that corrects for the influence of scattering and absorption on fluorescence intensity (F(SF)). The model expresses F(SF) in terms of the reduced scattering coefficient (μs') and absorption coefficient (μ(a)), each determined independently at excitation and emission wavelengths (λ(x) and λ(m)), and the fiber diameter (d(f)). This model returns accurate descriptions (mean residual <6%) of F(SF) across a biologically relevant range of μs' and μ(a) values and is insensitive to the form of the scattering phase function.
本研究利用单纤维荧光的蒙特卡罗模拟，开发了一种经验模型，用于校正散射和吸收对荧光强度（F(SF)）的影响。该模型以在激发和发射波长（λ(x)和λ(m)）下独立确定的降低散射系数（μs'）和吸收系数（μ(a)）以及纤维直径（d(f)）来表示 F(SF)。该模型在具有生物学相关性的μs'和μ(a)值范围内返回了对 F(SF)的准确描述（平均残余<6%），并且对散射相位函数的形式不敏感。
