Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
J Clin Microbiol. 2012 May;50(5):1755-7. doi: 10.1128/JCM.00132-12. Epub 2012 Feb 29.
We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.
我们应用 MIRU-VNTR(分枝杆菌间隔重复单元可变数目串联重复分型)直接分析来自肺结核患者的 61 份阳性培养物标本中的细菌。除了一个标本(一个位点无扩增)外(98.4%),所有标本均获得完整的 MIRU 型(24 个位点),等位基因值与相应培养物完全一致。我们的研究首次证明可以实现结核分枝杆菌实时基因分型,这完全改变了分子流行病学技术整合到控制计划中的方式。