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本文引用的文献

1
Innovations in the molecular epidemiology of tuberculosis.结核病分子流行病学的创新。
Enferm Infecc Microbiol Clin. 2011 Mar;29 Suppl 1:8-13. doi: 10.1016/S0213-005X(11)70012-X.
2
Advanced survey of tuberculosis transmission in a complex socioepidemiologic scenario with a high proportion of cases in immigrants.在移民病例比例较高的复杂社会流行病学情景下对结核病传播的深入调查。
Clin Infect Dis. 2008 Jul 1;47(1):8-14. doi: 10.1086/588785.
3
Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis.评估优化的分枝杆菌散布重复单位可变数目串联重复序列分型系统联合间隔寡核苷酸分型技术用于基于人群的结核病分子流行病学研究
J Clin Microbiol. 2007 Mar;45(3):691-7. doi: 10.1128/JCM.01393-06. Epub 2006 Dec 27.
4
Proposal for standardization of optimized mycobacterial interspersed repetitive unit-variable-number tandem repeat typing of Mycobacterium tuberculosis.结核分枝杆菌优化的分枝杆菌插入重复单位-可变数目串联重复序列分型标准化方案。
J Clin Microbiol. 2006 Dec;44(12):4498-510. doi: 10.1128/JCM.01392-06. Epub 2006 Sep 27.
5
A 13-year molecular epidemiological analysis of tuberculosis in San Francisco.旧金山结核病的13年分子流行病学分析。
Int J Tuberc Lung Dis. 2006 Mar;10(3):297-304.
6
Automated high-throughput genotyping for study of global epidemiology of Mycobacterium tuberculosis based on mycobacterial interspersed repetitive units.基于分枝杆菌散布重复单位的用于结核分枝杆菌全球流行病学研究的自动化高通量基因分型
J Clin Microbiol. 2001 Oct;39(10):3563-71. doi: 10.1128/JCM.39.10.3563-3571.2001.

实时分子流行病学研究通过直接对涂阳临床标本进行基因分型诊断结核病。

Real-time molecular epidemiology of tuberculosis by direct genotyping of smear-positive clinical specimens.

机构信息

Servicio de Microbiología y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain.

出版信息

J Clin Microbiol. 2012 May;50(5):1755-7. doi: 10.1128/JCM.00132-12. Epub 2012 Feb 29.

DOI:10.1128/JCM.00132-12
PMID:22378907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3347103/
Abstract

We applied MIRU-VNTR (mycobacterial interspersed repetitive-unit-variable-number tandem-repeat typing) to directly analyze the bacilli present in 61 stain-positive specimens from tuberculosis patients. A complete MIRU type (24 loci) was obtained for all but one (no amplification in one locus) of the specimens (98.4%), and the allelic values fully correlated with those obtained from the corresponding cultures. Our study is the first to demonstrate that real-time genotyping of Mycobacterium tuberculosis can be achieved, fully transforming the way in which molecular epidemiology techniques can be integrated into control programs.

摘要

我们应用 MIRU-VNTR（分枝杆菌间隔重复单元可变数目串联重复分型）直接分析来自肺结核患者的 61 份阳性培养物标本中的细菌。除了一个标本（一个位点无扩增）外（98.4%），所有标本均获得完整的 MIRU 型（24 个位点），等位基因值与相应培养物完全一致。我们的研究首次证明可以实现结核分枝杆菌实时基因分型，这完全改变了分子流行病学技术整合到控制计划中的方式。