结核临床样本直接基因分型的利弊

Pros and cons of direct genotyping on tuberculosis clinical samples.

作者信息

Sadegh Hamidreza, Kargarpour Kamakoli Mansour, Farmanfarmaei Ghazaleh, Masoumi Morteza, Abdolrahimi Farid, Fateh Abolfazl, Ebrahimzadeh Nayereh, Rahimi Jamnani Fatemeh, Vaziri Farzam, Siadat Seyed Davar

机构信息

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.

出版信息

Microb Pathog. 2017 Feb;103:135-138. doi: 10.1016/j.micpath.2016.12.025. Epub 2016 Dec 26.

DOI:10.1016/j.micpath.2016.12.025

PMID:28034830

Abstract

OBJECTIVE

Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB).

METHODS

We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-repeat) directly on 135 clinical samples from 84 TB patients.

RESULTS

There was a direct correlation between genotyping on clinical samples by MIRU-VNTR and bacterial load (P = 0.001). VNTR loci were amplified successfully for 41.5% of the clinical samples (19-24 loci), 32.6% (13-18 loci), 23.7% (7-12 loci) and 2.2% (1-6 loci). Loci of 2401, 577, 2996 and 154 had the highest power to show the mixed strains infection in clinical samples.

CONCLUSIONS

Direct MIRU-VNTR is partially successful in complete genotyping of M. tuberculosis strains. On the other hand, detection of polyclonal infection is undoubtedly reliable based on the direct MIRU-VNTR.

摘要

目的

快速对结核分枝杆菌进行基因分型对于改进结核病的分子流行病学调查至关重要。

方法

我们开展了一项回顾性研究，以评估直接在来自84例结核病患者的135份临床样本上使用24个位点的MIRU-VNTR（分枝杆菌散布重复单位-可变数目串联重复序列）的情况。

结果

通过MIRU-VNTR对临床样本进行基因分型与细菌载量之间存在直接相关性（P = 0.001）。VNTR位点在41.5%的临床样本（19 - 24个位点）、32.6%（13 - 18个位点）、23.7%（7 - 12个位点）和2.2%（1 - 6个位点）中成功扩增。2401、577、2996和154位点在显示临床样本中混合菌株感染方面具有最高效力。