Gordon Anita N, Marbach Cameron R, Oakey Jane, Edmunds Glen, Condon Kelly, Diviney Sinead M, Williams David T, Bingham John
Biosecurity Sciences Laboratory, Department of Employment, Economic Development and Innovation, Coopers Plains, Queensland, Australia.
J Vet Diagn Invest. 2012 Mar;24(2):431-6. doi: 10.1177/1040638711433325.
A 5-year-old Australian stock horse in Monto, Queensland, Australia, developed neurological signs and was euthanized after a 6-day course of illness. Histological examination of the brain and spinal cord revealed moderate to severe subacute, nonsuppurative encephalomyelitis. Sections of spinal cord stained positively in immunohistochemistry with a flavivirus-specific monoclonal antibody. Reverse transcription polymerase chain reaction assay targeting the envelope gene of flavivirus yielded positive results from brain, spinal cord, cerebrospinal fluid, and facial nerve. A flavivirus was isolated from the cerebrum and spinal cord. Nucleotide sequences obtained from amplicons from both tissues and virus isolated in cell culture were compared with those in GenBank and had 96-98% identity with Murray Valley encephalitis virus. The partial envelope gene sequence of the viral isolate clustered into genotype 1 and was most closely related to a previous Queensland isolate.
澳大利亚昆士兰州蒙托的一匹5岁澳大利亚役用马出现神经症状，病程6天后实施了安乐死。对其脑和脊髓进行组织学检查发现中度至重度亚急性非化脓性脑脊髓炎。脊髓切片在免疫组织化学中用黄病毒特异性单克隆抗体染色呈阳性。针对黄病毒包膜基因的逆转录聚合酶链反应检测在脑、脊髓、脑脊液和面神经中均得到阳性结果。从大脑和脊髓中分离出一种黄病毒。将从这两个组织的扩增子以及细胞培养中分离出的病毒获得的核苷酸序列与GenBank中的序列进行比较，与墨累谷脑炎病毒有96% - 98%的同一性。病毒分离株的部分包膜基因序列聚类为基因型1，与先前的昆士兰分离株关系最为密切。
