Liu Mi, Chang Xiao-Rong, Yan Jie, Yi Shou-Xiang, Lin Ya-Ping, Yue Zeng-Hui, Peng Yan, Zhang Hong
Hong College of Acu-moxibustion and Tuina, Hunan University of Chinese Medicine, Changsha 410208, China.
Zhen Ci Yan Jiu. 2011 Dec;36(6):403-8.
To observe the effects of moxibustion on contents of epidermal growth factor (EGF) and transforming growth factor-alpha (TGF-alpha) and epidermal growth factor receptor (EGFR) expression in the gastric mucosa tissue in rats with gastric mucosal lesion.
Thirty-six SD rats were randomly and equally divided into control, model and moxibustion groups. Gastric mucosal lesion model was duplicated by restraint and cool water immersion stress. Pre-moxibustion was applied to "Zusanli" (ST 36) and "Zhongwan" (CV 12), "Pishu" (BL 20) and "Weishu" (BL 21) alternately, once everyday for 8 days before modeling. The contents of EGF and TGF-alpha in gastric mucosa were detected by enzyme-linked immunosorbent assay (ELISA) and the expression of EGFR determined by immunohistochemistry.
Compared with the control group, only TGF-alpha content in the gastric mucosa in the model group was increased significantly (P < 0.05). Compared with the model group, the EGF and TGF-alpha contents and EGFR immunoactivity in the gastric mucosa were increased significantly in the moxibustion group (P < 0.05, P < 0.01).
Pre-moxibustion at ST 36, CV 12, BL 20 and BL 21 can up-regulate gastric mucosal EGF and TGF-alpha contents and EGFR protein expression in gastric mucosa lesion rats, which may contribute to its effect in relieving stress-induced gastric mucosal injury.
观察艾灸对胃黏膜损伤大鼠胃黏膜组织中表皮生长因子（EGF）、转化生长因子-α（TGF-α）含量及表皮生长因子受体（EGFR）表达的影响。
将36只SD大鼠随机均分为对照组、模型组和艾灸组。采用束缚及冷水浸应激法复制胃黏膜损伤模型。造模前，交替艾灸“足三里”（ST 36）、“中脘”（CV 12）、“脾俞”（BL 20）和“胃俞”（BL 21），每日1次，共8天。采用酶联免疫吸附测定（ELISA）法检测胃黏膜中EGF和TGF-α的含量，免疫组织化学法检测EGFR的表达。
与对照组比较，模型组胃黏膜中仅TGF-α含量显著升高（P<0.05）。与模型组比较，艾灸组胃黏膜中EGF、TGF-α含量及EGFR免疫活性显著升高（P<0.05，P<0.01）。
艾灸ST 36、CV 12、BL 20和BL 21可上调胃黏膜损伤大鼠胃黏膜中EGF、TGF-α含量及EGFR蛋白表达，这可能是其缓解应激性胃黏膜损伤的作用机制。
