Yang Jing, Wang Chi
Department of Gastroenterology, First Hospital of Peking University, Beijing 100034, China.
Zhen Ci Yan Jiu. 2011 Dec;36(6):423-7.
To observe the influence of electroacupuncture (EA) of "Zusanli" (ST 36) and ST 36 + "Neiguan" (PC 6) on gastric distention induced transient lower esophageal sphincter relaxation (TLESR) in cats.
Forty cats were randomly divided into model (gastric distension) group, EA-ST 36 group, EA-PC 6 group, EA-ST 36 + PC 6 group, EA-non-acupoint group, EA-ST 36 + saline (2 mL i.v.) group, EA-ST 36 + L-arginine (precusor of nitric oxide, 200 mg/kg i.v.) group, and EA-ST 36+Tacrine (cholinesterase inhibitor, 5.6 mg/kg i. p.) group, with 5 cats being in each group. TLESR model was established by extending the stomach with an air balloon. EA (2 Hz/100 Hz, 3-4 mA) was applied to bilateral ST 36, PC 6 and ST 36 + PC 6 and non-acupoint (exterior-superior site of the hip) while gastric distension. L-Arginine was given 10 min before EA, and Tacrine was given 30 min before EA. The frequency of TLESR and lower esophageal sphincter pressure (LESP) were recorded by using a perfused sleeve assembly.
Compared with the model group, the frequency of TLESR in 60 min as well as the percentage of common cavity during TLESR were significantly decreased in the EA-ST 36, EA-PC 6, EA-ST 36 + PC 6, EA-ST 36 + saline groups (P < 0.05). Compared with the EA-ST 36 + saline group, the frequency of TLESR in 60 min was up-regulated significantly in the EA-ST 36 + Tacrine group (P < 0.01) rather than in the EA-ST 36 + L-Arginine group, suggesting a reduction of the efficacy of EA-ST 36 by cholinergic suppression (not by nitric oxide). No significant differences were found among the EA-ST 36, EA-PC 6 and EA-ST 36 + PC 6 groups in the frequency of TLESR and the percentage of common cavity (P > 0.05). In comparison with the baseline level, LESP was increased significantly only in the EA-ST 36 group (P < 0.05).
EA at ST 36 can reduce both the frequency of TLESR and the percentage of common cavity during TLESR in gastric distension cats, which may be mediated by cholinergic pathway.
观察针刺“足三里”（ST36）及ST36 + “内关”（PC6）对猫胃扩张诱发的一过性下食管括约肌松弛（TLESR）的影响。
40只猫随机分为模型（胃扩张）组、电针ST36组、电针PC6组、电针ST36 + PC6组、电针非穴位组、电针ST36 + 生理盐水（静脉注射2 mL）组、电针ST36 + L-精氨酸（一氧化氮前体，静脉注射200 mg/kg）组和电针ST36 + 他克林（胆碱酯酶抑制剂，腹腔注射5.6 mg/kg）组，每组5只。通过气囊扩张胃建立TLESR模型。在胃扩张时，对双侧ST36、PC6、ST36 + PC6及非穴位（髋部外上部位）施加电针（2 Hz/100 Hz，3 - 4 mA）。L-精氨酸在电针前10分钟给予，他克林在电针前30分钟给予。使用灌注袖套组件记录TLESR频率和下食管括约肌压力（LESP）。
与模型组比较，电针ST36组、电针PC6组、电针ST36 + PC6组、电针ST36 + 生理盐水组胃扩张60分钟内TLESR频率及TLESR期间共同腔百分比均显著降低（P < 0.05）。与电针ST36 + 生理盐水组比较，电针ST36 + 他克林组胃扩张60分钟内TLESR频率显著上调（P < 0.01），而电针ST36 + L-精氨酸组未上调，提示胆碱能抑制（而非一氧化氮）降低了电针ST36的疗效。电针ST36组、电针PC6组和电针ST36 + PC6组TLESR频率及共同腔百分比比较，差异无统计学意义（P > 0.05）。与基础水平比较，仅电针ST36组LESP显著升高（P < 0.05）。
针刺ST36可降低胃扩张猫TLESR频率及TLESR期间共同腔百分比，其机制可能与胆碱能通路有关。
