Zhang Delong, Wang Xiaodong, Lu Ruili, Li Kangle, Hu Fenglin
Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China.
Wei Sheng Wu Xue Bao. 2011 Dec;51(12):1616-24.
To determine the volatile components of mycelia of Isaria cateinannulata cultured under different culture conditions, and to analyze the relationships between the culture conditions and volatile metabolites.
Mycelia were cultured in solid plates with SDAY medium and liquid shake flasks with SDY medium. The culture conditions were at 25 degrees C and 8 days. Volatile components in the mycelia of I. cateinannulata were extracted with simultaneous distillation extraction and analyzed by gas chromatography-mass spectrometry.
Alkenes, alkanes, heterocyclic and polycyclic aromatic hydrocarbons (PAH) were existed abundantly both in the mycelia of liquid and solid cultures, but the kinds and relative concentrations of the volatile components in mycelia of liquid and solid cultures were very different. Forty-one compounds were identified from the mycelia of solid culture and 32 compounds were identified from the mycelia of liquid culture. Esters, quinones and oximes were only found in solid cultured mycelia whereas carboxylic acids were only discovered in the mycelia of liquid culture. At the same time, mycelia of liquid culture contained much more phenols. The most abundant compounds in mycelia of liquid and solid cultures were hydrocarbons. The volatile extracts of solid cultured mycelia contained 57.6% alkenes and 9.19% alkanes. The volatile extracts of liquid cultured mycelia contained 7.85% alkenes and 22.4% alkanes.
Liquid or solid culture conditions influenced the volatile components of mycelia of I. cateinannulata.
测定不同培养条件下培养的茶褐棒束孢菌丝体的挥发性成分，并分析培养条件与挥发性代谢产物之间的关系。
将菌丝体接种于含SDAY培养基的固体平板和含SDY培养基的液体摇瓶中。培养条件为25℃，培养8天。采用同时蒸馏萃取法提取茶褐棒束孢菌丝体中的挥发性成分，并用气相色谱-质谱联用仪进行分析。
液体培养和固体培养的菌丝体中均大量存在烯烃、烷烃、杂环和多环芳烃（PAH），但液体培养和固体培养的菌丝体中挥发性成分的种类和相对浓度差异很大。从固体培养的菌丝体中鉴定出41种化合物，从液体培养的菌丝体中鉴定出32种化合物。酯类、醌类和肟类仅在固体培养的菌丝体中发现，而羧酸类仅在液体培养的菌丝体中发现。同时，液体培养的菌丝体中酚类物质含量更多。液体培养和固体培养的菌丝体中含量最丰富的化合物是烃类。固体培养的菌丝体挥发性提取物中烯烃含量为57.6%，烷烃含量为9.19%。液体培养的菌丝体挥发性提取物中烯烃含量为7.85%，烷烃含量为22.4%。
液体或固体培养条件影响茶褐棒束孢菌丝体的挥发性成分。
