Zhang Delong, Li Shulin, Lu Ruili, Li Kangle, Luo Feifei, Peng Fan, Hu Fenglin
Anhui Provincial Key Laboratory of Microbial Control, Anhui Agricultural University, Hefei 230036, China.
Wei Sheng Wu Xue Bao. 2012 Dec 4;52(12):1477-88.
To determine the secondary metabolites production in mycelia of Paecilomyces militaris.
Mycelia were cultured in plates with sabouraud dextrose agar yeast medium at 25 degrees C for 9 days. Sampling was done every day from the second to the ninth day. The secondary metabolites in the mycelia of Paecilomyces militaris were extracted with either methanol or ethyl acetate. The extracts were blended and analyzed by liquid chromatography-mass spectrometry (LC-MS). LC-MS data were collected and analyzed by MetaboAnalyst software.
Principal component analysis indicates different secondary metabolites accumulation with incubation times. Hierarchical clustering analysis shows that the metabolic process of cationic compounds such as alkaloids, peptides and nucleosides can be divided into three stages, and that the metabolic process of anionic compounds such as organic acids and saccharides can be divided into two stages. Metabolites difference and heat map analysis show that: (1) The number of metabolites with significant increased contents was raised significantly in mycelia of Paecilomyces militaris on the second and third incubation days. The main species with increased contents were esters and their hydrolized products, destruxin B, variotin and some unidentified nitrogin contained compounds. (2) The number of metabolites with significant raised contents was decreased significantly on the fourth and fifth incubation days. The main species with increased contents were ophiocordin and destruxin A. (3) Apart from peptide antibiotics such as several beauverolides, the content increased metabolites included also several organic acids, amino acids, rhamnose, trehalose, cerebroside and riboflavine during the sixth to ninth incubation days.
The secondary metabolites in mycelia of Paecilomyces militaris were related significantly to the incubation time.
测定 militaris拟青霉菌丝体中次生代谢产物的产生情况。
将菌丝体接种于含有沙氏葡萄糖琼脂酵母培养基的平板中，在25℃下培养9天。从第二天到第九天每天进行取样。用甲醇或乙酸乙酯提取 militaris拟青霉菌丝体中的次生代谢产物。提取物经混合后通过液相色谱 - 质谱联用仪（LC - MS）进行分析。LC - MS数据由MetaboAnalyst软件收集并分析。
主成分分析表明，次生代谢产物的积累随培养时间而不同。层次聚类分析显示，生物碱、肽和核苷等阳离子化合物的代谢过程可分为三个阶段，而有机酸和糖类等阴离子化合物的代谢过程可分为两个阶段。代谢产物差异和热图分析表明：（1）在培养的第二天和第三天，militaris拟青霉菌丝体中含量显著增加的代谢产物数量显著上升。含量增加的主要种类为酯类及其水解产物、环孢素B、帚曲菌素以及一些未鉴定的含氮化合物。（2）在培养的第四天和第五天，含量显著上升的代谢产物数量显著下降。含量增加的主要种类为蛇形菌素和环孢素A。（3）在培养的第六天至第九天，除了几种白僵菌素等肽类抗生素外，含量增加的代谢产物还包括几种有机酸、氨基酸、鼠李糖、海藻糖、脑苷脂和核黄素。
militaris拟青霉菌丝体中的次生代谢产物与培养时间显著相关。
