Exp Dermatol. 2012 Mar;21(3):221-3. doi: 10.1111/j.1600-0625.2011.01423.x.
Considering the clinical and genetic heterogeneity of psoriasis which may translate into distinct disease pathology and treatment response, correct typing of the main candidate gene HLA-C is critical but not trivial. To facilitate genotyping, we compared established techniques with our newly developed tool. Here, we propose that typing of four single nucleotide polymorphic markers within the HLA-C region correctly determines HLA-Cw*06:02 genotypes in psoriatic cases and healthy controls in a population of Caucasian origin. Typing of the SNPs presented herein proved to be precise, reliable, time and cost effective, and requiring low amount of DNA.
鉴于银屑病的临床和遗传异质性可能转化为不同的疾病病理和治疗反应，正确确定主要候选基因 HLA-C 的类型至关重要，但并非微不足道。为了便于基因分型，我们将已建立的技术与我们新开发的工具进行了比较。在这里，我们提出在一个白种人群体中，对 HLA-C 区域内的四个单核苷酸多态性标记进行分型，可以正确确定银屑病病例和健康对照者中的 HLA-Cw*06:02 基因型。本文所述 SNP 的分型被证明是精确、可靠、省时、省钱且需要的 DNA 量低。
