Hasan Mahmudul, Islam Mohammed Mafizul, Khan Mukhlesur Rahman, Alam Mohammad Shafiqul, Kurabayashi Atsushi, Igawa Takeshi, Kuramoto Mitsuru, Sumida Masayuki
Institute for Amphibian Biology, Hiroshima University, Higashihiroshima, Japan.
Zoolog Sci. 2012 Mar;29(3):162-72. doi: 10.2108/zsj.29.162.
To survey the diversity of anuran species in Bangladesh, we compared mitochondrial 16S rRNA gene sequences (approximately 1.4 kbp) from 107 Bangladesh frog specimens. The results of genetic divergence and phylogenetic analyses incorporating data from related species revealed the occurrence of at least eight cryptic species. Hoplobatrachus tigerinus from two districts diverged considerably, indicating the involvement of a cryptic species. Two Fejervarya sp. (large and medium types) and Hylarana cf. taipehensis formed lineages distinct from related species and are probably new species. Microhyla cf. ornata differed from M. ornata with respect to type locality area and involved two distinct species. In addition, we found that Hylarana sp. and Microhyla sp. did not match congeners examined to date in either morphology or 16S rRNA sequence. The occurrence of M. fissipes was tentatively suggested. Consequently, at least, 19 species were found from Bangladesh in this study. These findings revealed a rich anuran biodiversity in Bangladesh, which is unexpected considering the rather simple topographic features of the country.
为了调查孟加拉国无尾目物种的多样性，我们比较了107个孟加拉国青蛙标本的线粒体16S rRNA基因序列（约1.4 kbp）。结合相关物种数据的遗传分化和系统发育分析结果显示，至少存在8个隐存种。来自两个地区的虎纹蛙差异很大，表明存在一个隐存种。两种姬蛙属物种（大型和中型）以及拟台北水蛙形成了与相关物种不同的谱系，可能是新物种。饰纹姬蛙在模式产地方面与饰纹姬蛙不同，涉及两个不同的物种。此外，我们发现某水蛙属物种和某姬蛙属物种在形态或16S rRNA序列上均与迄今所研究的同属物种不匹配。初步推测有泽蛙的存在。因此，本研究中从孟加拉国至少发现了19个物种。这些发现揭示了孟加拉国丰富的无尾目生物多样性，考虑到该国相对简单的地形特征，这是出乎意料的。
