Satoh Akira, Hirata Ayako, Makanae Aki
Okayama University, Research Core of Interdisciplinary Sciences, Kitaku, Okayama, Japan.
Zoolog Sci. 2012 Mar;29(3):191-7. doi: 10.2108/zsj.29.191.
Amphibians can regenerate missing body parts, including limbs. The regulation of collagen has been considered to be important in limb regeneration. Collagen deposition is suppressed during limb regeneration, so we investigated collagen deposition and apical epithelial cap (AEC) formation during axolotl limb regeneration. The accessory limb model (ALM) has been developed as an alternative model for studying limb regeneration. Using this model, we investigated the relationship between nerves, epidermis, and collagen deposition. We found that Sp-9, an AEC marker gene, was upregulated by direct interaction between nerves and epidermis. However, collagen deposition hindered this interaction, and resulted in the failure of limb regeneration. During wound healing, an increase in deposition of collagen caused a decrease in the blastema induction rate in ALM. Wound healing and limb regeneration are alternate processes.
两栖动物能够再生缺失的身体部位，包括四肢。胶原蛋白的调节被认为在肢体再生中很重要。在肢体再生过程中，胶原蛋白沉积受到抑制，因此我们研究了蝾螈肢体再生过程中的胶原蛋白沉积和顶端上皮帽（AEC）形成。辅助肢体模型（ALM）已被开发出来作为研究肢体再生的替代模型。利用这个模型，我们研究了神经、表皮和胶原蛋白沉积之间的关系。我们发现，作为AEC标记基因的Sp-9通过神经与表皮之间的直接相互作用而上调。然而，胶原蛋白沉积阻碍了这种相互作用，并导致肢体再生失败。在伤口愈合过程中，胶原蛋白沉积的增加导致了ALM中芽基诱导率的降低。伤口愈合和肢体再生是交替进行的过程。
