Lim F T, Scherjon S A, van Beckhoven J M, Brand A, Kanhai H H, Hermans J M, Falkenburg J H
Department of Hematology, Leiden University Medical Center, Leiden, The Netherlands.
Am J Obstet Gynecol. 2000 Nov;183(5):1144-52. doi: 10.1067/mob.2000.108848.
Umbilical cord blood can be used as a source of bone marrow repopulating cells for allogeneic stem cell transplantation. Large variations in the frequencies of white blood cells and hematopoietic progenitor cells have been found for umbilical cord blood. These variations may be due in part to specific circumstances during labor and delivery.
In this study we analyzed the relationship between stress factors occurring during parturition and the frequencies of nucleated cells, leukocyte subsets, CD34(+) cells, and hematopoietic progenitor cells, as determined in semisolid medium cultures of umbilical cord blood.
We observed that a prolonged first stage of labor resulted in increases in the numbers of nucleated cells, granulocytes, CD34(+) cells, and hematopoietic progenitor cells in umbilical cord blood. Evaluation of parameters that indicate stress of the infant during delivery demonstrated higher numbers of nucleated cells, granulocytes, CD34(+) cells, and hematopoietic progenitor cells in umbilical cord blood from children with lower venous pH.
Longer duration stress during delivery increased the numbers of nucleated cells, granulocytes, CD34(+) cells, and hematopoietic progenitor cells, possibly by causing mobilization of various cell populations by endogenous cytokines. As long as umbilical cord blood harvesting does not interfere with the delivery, umbilical cord blood collected after stressful deliveries may provide optimal units for hematopoietic stem cell transplantation.
脐带血可作为异基因干细胞移植的骨髓重建细胞来源。已发现脐带血中白细胞和造血祖细胞的频率存在很大差异。这些差异可能部分归因于分娩过程中的特定情况。
在本研究中，我们分析了分娩期间出现的应激因素与脐带血半固体培养基培养中确定的有核细胞、白细胞亚群、CD34(+)细胞和造血祖细胞频率之间的关系。
我们观察到第一产程延长导致脐带血中有核细胞、粒细胞、CD34(+)细胞和造血祖细胞数量增加。对表明婴儿分娩时应激的参数进行评估发现，静脉血pH值较低的儿童脐带血中有核细胞、粒细胞、CD34(+)细胞和造血祖细胞数量较多。
分娩期间较长时间的应激增加了有核细胞、粒细胞、CD34(+)细胞和造血祖细胞的数量，可能是通过内源性细胞因子促使各种细胞群体动员所致。只要脐带血采集不干扰分娩，在应激分娩后采集的脐带血可能为造血干细胞移植提供最佳样本。
