Surbek D V, Danzer E, Steinmann C, Tichelli A, Wodnar-Filipowicz A, Hahn S, Holzgreve W
Department of Obstetrics and Gynecology, University of Basel, Switzerland.
Am J Obstet Gynecol. 2001 Sep;185(3):725-9. doi: 10.1067/mob.2001.117343.
The aim of the present study was to determine the influence of preeclampsia on cord blood hematopoietic progenitor-stem cells obtained at delivery because cord blood is increasingly used clinically for stem cell retrieval as an alternative to bone marrow.
Umbilical cord blood was collected from patients fulfilling the criteria for preeclampsia and from gestational age- and birth weight-matched control subjects at delivery (patient/control subjects ratio, 1:2). Cord blood volume and nucleated cell content were measured, and the number of hematopoietic progenitor-stem cells was determined by means of fluorescence-activated cell sorting with the CD34(+) epitope and by means of colony assays with different hematopoietic growth factors. In addition, the expression of adhesion molecules by CD34(+) progenitor-stem cells was examined.
In pregnancies affected by preeclampsia, volume and nucleated cell and total CD34(+) cell contents in the collected cord blood were significantly smaller compared with those of control subjects. Furthermore, there was a trend toward a smaller relative number of CD34(+) cells and colony-forming units per nucleated cell in cord blood samples from preeclamptic patients. No difference in the expression of the cell-adhesion molecules leukocyte function-associated antigen 1, very late activation antigen 4, and L-selectin by CD34(+) cells could be found.
This study shows that preeclampsia affects umbilical cord blood volume and nucleated cell and progenitor-stem cell numbers obtained at birth.
本研究旨在确定子痫前期对分娩时获得的脐血造血祖干细胞的影响，因为脐血在临床上越来越多地被用作替代骨髓来获取干细胞。
在分娩时，从符合子痫前期标准的患者以及孕周和出生体重匹配的对照受试者中采集脐带血（患者/对照受试者比例为1:2）。测量脐带血体积和有核细胞含量，并通过使用CD34(+)表位的荧光激活细胞分选法以及使用不同造血生长因子的集落测定法来确定造血祖干细胞的数量。此外，检测CD34(+)祖干细胞上黏附分子的表达。
在受子痫前期影响的妊娠中，所采集脐带血的体积、有核细胞和总CD34(+)细胞含量与对照受试者相比明显较少。此外，子痫前期患者脐带血样本中每有核细胞的CD34(+)细胞和集落形成单位的相对数量有减少的趋势。未发现CD34(+)细胞上白细胞功能相关抗原1、极晚期活化抗原4和L-选择素等细胞黏附分子的表达有差异。
本研究表明，子痫前期会影响出生时获得的脐带血体积、有核细胞数量和祖干细胞数量。
