Sergeenko N G, Sal'nikov K V, Lebedev O E
Tsitologiia. 1990;32(4):390-4.
Further investigation of the peculiarities of flavin fluorescence photodestruction in malignant cells was made. In normal cells incubated in low pH (3.0-3.2) physiological solutions, the decrease in the oxidized flavoprotein fluorescence intensity under irradiation is the same as in normal pH condition, whereas in tumor cell in low pH solutions a significant increase in the photodestruction level was noticed. The cells isolated from foci of transformation, following treatment of 3T3 NIH fibroblasts with the carcinogen N-methyl-N'-nitro-N-nitrosoguanidine, displayed the photodestruction parameters similar to those in tumor cells. A two-step analysis of cells is proposed for distinguishing between the normal and malignant cells.
对恶性细胞中黄素荧光光破坏特性进行了进一步研究。在低pH（3.0 - 3.2）生理溶液中孵育的正常细胞，照射下氧化黄素蛋白荧光强度的降低与正常pH条件下相同，而在低pH溶液中的肿瘤细胞中，光破坏水平显著增加。在用致癌物N - 甲基 - N' - 硝基 - N - 亚硝基胍处理3T3 NIH成纤维细胞后，从转化灶分离出的细胞显示出与肿瘤细胞相似的光破坏参数。提出了一种用于区分正常细胞和恶性细胞的细胞两步分析法。
