凝血酶可诱导人脑血管内皮细胞系呈现炎症表型。
Thrombin induces an inflammatory phenotype in a human brain endothelial cell line.
机构信息
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
出版信息
J Neuroimmunol. 2012 Apr;245(1-2):48-55. doi: 10.1016/j.jneuroim.2012.02.004. Epub 2012 Feb 29.
Abstract
In this study, we utilized the human brain endothelial cell line, hCMEC/D3, to determine the effects of the coagulation factor, thrombin, on the human blood-brain barrier (BBB). We show that thrombin increased the mRNA and cell surface levels of ICAM-1 and VCAM-1 in hCMEC/D3 cells. Thrombin similarly upregulated several chemokines implicated in human neurological conditions. Additionally, the paracellular permeability of the human BBB in vitro was also increased following thrombin treatment. Overall, this study demonstrates that thrombin can effectively induce an inflamed phenotype in an in vitro human BBB.
摘要
在这项研究中，我们利用人脑血管内皮细胞系 hCMEC/D3 来确定凝血因子凝血酶对人血脑屏障（BBB）的影响。我们发现凝血酶增加了 hCMEC/D3 细胞中 ICAM-1 和 VCAM-1 的 mRNA 和细胞表面水平。凝血酶同样上调了几种与人类神经状况有关的趋化因子。此外，凝血酶处理后体外人 BBB 的细胞旁通透性也增加。总的来说，这项研究表明凝血酶可以在体外有效地诱导人 BBB 产生炎症表型。
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