Konovalenko O V, Maglysh S S, Gorbach Z V
Ukr Biokhim Zh (1978). 1990 Jul-Aug;62(4):50-5.
Thiamine-deficiency in animals induced by everyday subcutaneous administration of oxythiamine in a dose of 4, 40 and 100 mg/kg of weight for 10 days results in a decrease of the total activity of pyruvate kinase in the liver tissue and does not affect the mentioned index in the kidney and heart tissues. It is shown that as a result of the enzyme fractionation in the column with DEAE-cellulose the total activity of pyruvate kinase in the liver tissue of rats with thiamine-deficiency decreases due to L-isoform while the content of M-isoform remains unchanged. Thiamine deficiency does not affect kinetic characteristics of the L-isoform, extracted from the liver and this shows the absence of changes in the degree of phosphorylation of pyruvate kinase L-isoform under these conditions.
每天皮下注射剂量为4、40和100毫克/千克体重的氧硫胺，持续10天，可诱导动物出现硫胺素缺乏，这会导致肝脏组织中丙酮酸激酶的总活性降低，而对肾脏和心脏组织中的上述指标没有影响。结果表明，通过用DEAE-纤维素柱对酶进行分级分离，硫胺素缺乏的大鼠肝脏组织中丙酮酸激酶的总活性因L-同工型降低，而M-同工型的含量保持不变。硫胺素缺乏不影响从肝脏中提取的L-同工型的动力学特性，这表明在这些条件下丙酮酸激酶L-同工型的磷酸化程度没有变化。
