Maslovskaya A A, Lukashik N K
Chair of Biochemistry, Medical Institute, Grodno, B. S. S. R., U. S. S. R.
Acta Biochim Pol. 1990;37(1):129-33.
Activity of the key enzymes of gluconeogenesis under alimentary thiamine deficiency (15 days of dietary treatment) was studied in the liver and kidney of fed and 48 h starved rats. As compared to pair-fed controls vitamin B1-deficiency was followed by a decrease of glucose 6-phosphatase and fructose 1,6-bisphosphatase activities in both organs; the activity of phosphoenolpyruvate carboxykinase was diminished only in the liver. Starvation of thiamine-deficient rats (as compared to pair-fed starved group) resulted in lower activation of these enzymes. The decrease of the enzyme activities in thiamine-deficient animals indicates that de novo glucose synthesis in the tissues is depressed, though thiamine-requiring enzymes are not directly involved in this process. Possible mechanisms of alterations described are discussed.
在喂食和饥饿48小时的大鼠的肝脏和肾脏中，研究了饮食性硫胺素缺乏（饮食治疗15天）情况下糖异生关键酶的活性。与配对喂食的对照组相比，维生素B1缺乏导致两个器官中葡萄糖6-磷酸酶和果糖1,6-二磷酸酶的活性降低；磷酸烯醇式丙酮酸羧激酶的活性仅在肝脏中降低。硫胺素缺乏大鼠的饥饿（与配对喂食的饥饿组相比）导致这些酶的激活程度较低。硫胺素缺乏动物中酶活性的降低表明组织中从头合成葡萄糖的过程受到抑制，尽管需要硫胺素的酶并不直接参与这一过程。文中讨论了所描述变化的可能机制。
